{"created":"2021-03-01T07:21:09.357819+00:00","id":41671,"links":{},"metadata":{"_buckets":{"deposit":"98c7fd8c-4623-433f-8e9e-7c95f6216564"},"_deposit":{"id":"41671","owners":[],"pid":{"revision_id":0,"type":"depid","value":"41671"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00041671","sets":["2780:1782","2780:918","3:62:5595:5975"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"18","bibliographicPageStart":"14","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Medical journal of Indonesia"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡邊, 幸秀"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"加藤, 光保"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background:\n Clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) is a powerful genome editing technique. It consists of RNA-guided DNA endonuclease Cas9 and single guide RNA (gRNA). By combining their expressions, high efficiency cleavage of the target gene can be achieved, leading to the formation of DNA double-strand break (DSB) at the genomic locus of interest which will be repaired via NHEJ (non-homologous end joining) or HDR (homology-directed repair) and mediate DNA alteration. We aimed to apply the CRISPR/Cas9 technique to knock-out the transmembrane prostate androgen-induced protein (TMEPAI) gene in the triple negative breast cancer cell line.\nMethods:\n Designed gRNA which targets the TMEPAI gene was synthesized, annealed, and cloned into gRNA expression vector. It was co-transfected into the TNBC cell line using polyethylenimine (PEI) together with Cas9-GFP and puromycin resistant gene vector. At 24-hours post-transfection, cells were selected by puromycin for 3 days before they were cloned. Selected knock-out clones were subsequently checked on their protein levels by western blotting.\nResults:\nCRISPR/Cas9, a genome engineering technique successfully knocked-out TMEPAI in the Hs578T TNBC cell line. Sequencing shows a frameshift mutation in TMEPAI. Western blot shows the absence of TMEPAI band on Hs578T KO cells.\nConclusion: \nTMEPAI gene was deleted in the TNBC cell line using the genomic editing technique CRISPR/Cas9. The deletion was confirmed by genome and protein analysis.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"University of Indonesia, Faculty of Medicine"}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.13181/mji.v26i1.1871","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright @ 2017 Authors."},{"subitem_rights":"This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are properly cited."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2252-8083","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1113872X","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wardhani, Bantari W.K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Puteri, Meidi U."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Yukihide"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Louisa, Melva"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Setiabudy, Rianto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Mitsuyasu"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-06-23"}],"displaytype":"detail","filename":"MJI_26-1.pdf","filesize":[{"value":"566.3 kB"}],"format":"application/pdf","licensetype":"license_9","mimetype":"application/pdf","url":{"label":"MJI_26-1","url":"https://tsukuba.repo.nii.ac.jp/record/41671/files/MJI_26-1.pdf"},"version_id":"faf3e71f-b592-4d06-831c-cd7dd088011d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"TMEPAI genome editing in triple negative breast cancer cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"TMEPAI genome editing in triple negative breast cancer cells"}]},"item_type_id":"5","owner":"1","path":["918","1782","5975"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-06-23"},"publish_date":"2017-06-23","publish_status":"0","recid":"41671","relation_version_is_last":true,"title":["TMEPAI genome editing in triple negative breast cancer cells"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:12:37.593194+00:00"}