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  1. 医学医療系 (Faculty of Medicine)
  2. 柴 綾 (Shiba Aya)
  1. 医学医療系 (Faculty of Medicine)
  2. 野口 雅之 (Noguchi Masayuki)
  1. 医学医療系 (Faculty of Medicine)
  2. 加野 准子 (Kano Junko)
  1. 医学医療系 (Faculty of Medicine)
  2. 坂下 信悟 (Sakashita Shingo)
  1. コンテンツタイプ (Contents Type)
  2. 雑誌発表論文等 (Journal article, etc.)
  3. C~
  4. Cancer science

miR-3941: A novel microRNA that controls IGBP1 expression and is associated with malignant progression of lung adenocarcinoma

http://hdl.handle.net/2241/00146023
5f591056-d792-4a80-8927-029a501d8621
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CS_108-3-536.pdf CS_108-3-536 (979.6 kB)
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item type Journal Article(1)
公開日 2017-05-01
タイトル
タイトル miR-3941: A novel microRNA that controls IGBP1 expression and is associated with malignant progression of lung adenocarcinoma
言語
言語 eng
資源タイプ
タイプ journal article
著者 Sato, Taiki

× Sato, Taiki

WEKO 161327

Sato, Taiki

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Shiba-Ishii, Aya

× Shiba-Ishii, Aya

WEKO 161328

Shiba-Ishii, Aya

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Kim, Yunjung

× Kim, Yunjung

WEKO 161329

Kim, Yunjung

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Dai, Tomoko

× Dai, Tomoko

WEKO 161330

Dai, Tomoko

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Husni, Ryan Edbert

× Husni, Ryan Edbert

WEKO 161331

Husni, Ryan Edbert

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Hong, JeongMin

× Hong, JeongMin

WEKO 161332

Hong, JeongMin

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Kano, Junko

× Kano, Junko

WEKO 161333

Kano, Junko

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Sakashita, Shingo

× Sakashita, Shingo

WEKO 161334

Sakashita, Shingo

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Iijima, Tatsuo

× Iijima, Tatsuo

WEKO 161335

Iijima, Tatsuo

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Noguchi, Masayuki

× Noguchi, Masayuki

WEKO 161336

Noguchi, Masayuki

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著者別名 柴, 綾

× 柴, 綾

WEKO 161337

柴, 綾

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加野, 准子

× 加野, 准子

WEKO 204176
e-Rad 60334059
筑波大学研究者総覧 0000001654

加野, 准子

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坂下, 信悟

× 坂下, 信悟

WEKO 161339

坂下, 信悟

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野口, 雅之

× 野口, 雅之

WEKO 124641
e-Rad 00198582
筑波大学研究者総覧 0000001620

野口, 雅之

Search repository
抄録
内容記述 Immunoglobulin (CD79a) binding protein 1 (IGBP1) is universally overexpressed in lung adenocarcinoma and exerts an anti-apoptotic effect by binding to PP2Ac. However, the molecular mechanism of IGBP1 overexpression is still unclear. In the present study, we used a microRNA (miRNA) array and TargetScan Human software to detect IGBP1-related miRNAs that regulate IGBP1 expression. The miRNA array analysis revealed more than 100 miRNAs that are dysregulated in early invasive adenocarcinoma. On the other hand, in silico analysis using TargetScan Human revealed 79 miRNAs that are associated with IGBP1 protein expression. Among the miRNAs selected by miRNA array analysis, six (miR-34b, miR-138, miR-374a, miR-374b, miR-1909, miR-3941) were also included among those selected by TargetScan analysis. Real-time reverse transcription PCR (real-time RT-PCR) showed that the six microRNAs were downregulated in invasive adenocarcinoma (IGBP1+) relative to adjacent normal lung tissue (IGBP1−). Among these microRNAs, only miR-34b and miR-3941 depressed luciferase activity by targeting 3′UTR-IGBP1 in the luciferase vector. We transfected miR-34b and miR-3941 into lung adenocarcinoma cell lines (A549, PC-9), and both of them suppressed IGBP1 expression and cell proliferation. Moreover, the transfected miR-34b and miR-3941 induced apoptosis of a lung adenocarcinoma cell line, similarly to the effect of siIGBP1 RNA. As well as miR-34b, we found that miR-3941 targeted IGBP1 specifically and was able to exclusively downregulate IGBP1 expression. These findings indicate that suppression of miR-3941 has an important role in the progression of lung adenocarcinoma at an early stage.
書誌情報 Cancer science

巻 108, 号 3, p. 536-542, 発行日 2017-03
ISSN
収録物識別子 13479032
書誌レコードID
収録物識別子 AA11808050
PubMed番号
関連識別子
関連識別子 28012229
DOI
関連識別子
関連識別子 10.1111/cas.13148
権利
権利情報 © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
権利
権利情報 This is an open access article under the terms of the Creative Commons Attrib ution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
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値 publisher
出版者
出版者 Wiley
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