{"_buckets": {"deposit": "d1b817b0-08f0-479c-839e-9da6f046d424"}, "_deposit": {"id": "41076", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "41076"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00041076"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "542", "bibliographicPageStart": "536", "bibliographicVolumeNumber": "108", "bibliographic_titles": [{"bibliographic_title": "Cancer science"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u67f4, \u7dbe"}], "nameIdentifiers": [{"nameIdentifier": "161337", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u52a0\u91ce, \u51c6\u5b50"}], "nameIdentifiers": [{"nameIdentifier": "204176", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60334059", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000060334059"}, {"nameIdentifier": "0000001654", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001654"}]}, {"creatorNames": [{"creatorName": "\u5742\u4e0b, \u4fe1\u609f"}], "nameIdentifiers": [{"nameIdentifier": "161339", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u91ce\u53e3, \u96c5\u4e4b"}], "nameIdentifiers": [{"nameIdentifier": "124641", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00198582", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000000198582"}, {"nameIdentifier": "0000001620", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000001620"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Immunoglobulin (CD79a) binding protein 1 (IGBP1) is universally overexpressed in lung adenocarcinoma and exerts an anti-apoptotic effect by binding to PP2Ac. However, the molecular mechanism of IGBP1 overexpression is still unclear. In the present study, we used a microRNA (miRNA) array and TargetScan Human software to detect IGBP1-related miRNAs that regulate IGBP1 expression. The miRNA array analysis revealed more than 100 miRNAs that are dysregulated in early invasive adenocarcinoma. On the other hand, in silico analysis using TargetScan Human revealed 79 miRNAs that are associated with IGBP1 protein expression. Among the miRNAs selected by miRNA array analysis, six (miR-34b, miR-138, miR-374a, miR-374b, miR-1909, miR-3941) were also included among those selected by TargetScan analysis. Real-time reverse transcription PCR (real-time RT-PCR) showed that the six microRNAs were downregulated in invasive adenocarcinoma (IGBP1+) relative to adjacent normal lung tissue (IGBP1\u2212). Among these microRNAs, only miR-34b and miR-3941 depressed luciferase activity by targeting 3\u2032UTR-IGBP1 in the luciferase vector. We transfected miR-34b and miR-3941 into lung adenocarcinoma cell lines (A549, PC-9), and both of them suppressed IGBP1 expression and cell proliferation. Moreover, the transfected miR-34b and miR-3941 induced apoptosis of a lung adenocarcinoma cell line, similarly to the effect of siIGBP1 RNA. As well as miR-34b, we found that miR-3941 targeted IGBP1 specifically and was able to exclusively downregulate IGBP1 expression. These findings indicate that suppression of miR-3941 has an important role in the progression of lung adenocarcinoma at an early stage.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "28012229", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1111/cas.13148", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2016 The Authors. Cancer Science published by John Wiley \u0026 Sons Australia, Ltd on behalf of Japanese Cancer Association."}, {"subitem_rights": "This is an open access article under the terms of the Creative Commons Attrib ution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes."}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13479032", "subitem_source_identifier_type": "ISSN"}]}, "item_5_source_id_9": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11808050", "subitem_source_identifier_type": "NCID"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": " \u533b\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sato, Taiki"}], "nameIdentifiers": [{"nameIdentifier": "161327", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiba-Ishii, Aya"}], "nameIdentifiers": [{"nameIdentifier": "161328", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kim, Yunjung"}], "nameIdentifiers": [{"nameIdentifier": "161329", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Dai, Tomoko"}], "nameIdentifiers": [{"nameIdentifier": "161330", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Husni, Ryan Edbert"}], "nameIdentifiers": [{"nameIdentifier": "161331", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hong, JeongMin"}], "nameIdentifiers": [{"nameIdentifier": "161332", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kano, Junko"}], "nameIdentifiers": [{"nameIdentifier": "161333", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakashita, Shingo"}], "nameIdentifiers": [{"nameIdentifier": "161334", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iijima, Tatsuo"}], "nameIdentifiers": [{"nameIdentifier": "161335", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Noguchi, Masayuki"}], "nameIdentifiers": [{"nameIdentifier": "161336", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "CS_108-3-536.pdf", "filesize": [{"value": "979.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_9", "mimetype": "application/pdf", "size": 979600.0, "url": {"label": "CS_108-3-536", "url": "https://tsukuba.repo.nii.ac.jp/record/41076/files/CS_108-3-536.pdf"}, "version_id": "0436c9cc-4d58-4e65-9914-92b7f7aa568b"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "miR-3941: A novel microRNA that controls IGBP1 expression and is associated with malignant progression of lung adenocarcinoma", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "miR-3941: A novel microRNA that controls IGBP1 expression and is associated with malignant progression of lung adenocarcinoma"}]}, "item_type_id": "5", "owner": "1", "path": ["2780/5781", "2780/5859", "2780/2611", "2780/2618", "3/62/5298/1032"], "permalink_uri": "http://hdl.handle.net/2241/00146023", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-05-01"}, "publish_date": "2017-05-01", "publish_status": "0", "recid": "41076", "relation": {}, "relation_version_is_last": true, "title": ["miR-3941: A novel microRNA that controls IGBP1 expression and is associated with malignant progression of lung adenocarcinoma"], "weko_shared_id": 5}