{"created":"2021-03-01T07:20:13.598123+00:00","id":40804,"links":{},"metadata":{"_buckets":{"deposit":"a817ce90-d975-4e76-9253-fc464626bb5d"},"_deposit":{"id":"40804","owners":[],"pid":{"revision_id":0,"type":"depid","value":"40804"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00040804","sets":["160:3113","160:519","3:62:5587:395"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"161","bibliographicPageStart":"153","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"Extremophiles"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"星野, 貴行"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中村, 顕"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"There are 12 putative toxin–antitoxin (TA) loci in the Thermus thermophilus HB27 genome, including four VapBC and three HicBA families. Expression of these seven putative toxin genes in Escherichia coli demonstrated that one putative VapC toxin TTC0125 and two putative HicA toxins, TTC1395 and TTC1705, inhibited cell growth, and co-expression with cognate antitoxin genes rescued growth, indicating that these genes function as TA loci. In vitro analysis with the purified TTC0125 and total RNA/mRNA from E. coli and T. thermophilus showed that TTC0125 has RNase activity to rRNA and mRNA; this activity was inhibited by the addition of the purified TTC0126. Translation inhibition assays showed that TTC0125 inhibited protein synthesis by degrading mRNA but not by inactivating ribosomes. Amino acid substitutions of 14 predicted catalytic and conserved residues in VapC toxins to Ala or Asp in TTC0125 indicated that nine residues are important for its in vivo toxin activity and in vitro RNase activity. These data demonstrate that TTC0125-TTC0126 functions as a VapBC TA module and causes growth inhibition by degrading free RNA. This is the first study to identify the function of TA systems in T. thermophilus.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":" Springer "}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27853887","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s00792-016-0891-1","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Springer Japan 2016"},{"subitem_rights":"The final publication is available at Springer via http://dx.doi.org/10.1007/s00792-016-0891-1"}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1431-0651","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11156867","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fan, Yuqi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hoshino, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Akira"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-01"}],"displaytype":"detail","filename":"Extremophiles_12-1.pdf","filesize":[{"value":"802.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Extremophiles_12-1","url":"https://tsukuba.repo.nii.ac.jp/record/40804/files/Extremophiles_12-1.pdf"},"version_id":"859480ab-05d8-4125-9e1a-d4f7a0589ee4"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-01"}],"displaytype":"detail","filename":"Extremophiles_12-1_suppl..pdf","filesize":[{"value":"435.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Extremophiles_12-1_suppl.","url":"https://tsukuba.repo.nii.ac.jp/record/40804/files/Extremophiles_12-1_suppl..pdf"},"version_id":"5ffda9ae-64e8-4dd7-aeac-4d64bca05b22"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27"}]},"item_type_id":"5","owner":"1","path":["519","3113","395"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-06"},"publish_date":"2017-04-06","publish_status":"0","recid":"40804","relation_version_is_last":true,"title":["Identification of a VapBC toxin–antitoxin system in a thermophilic bacterium Thermus thermophilus HB27"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:11:20.165142+00:00"}