@article{oai:tsukuba.repo.nii.ac.jp:00040577,
 author = {吉田, 昌樹 and 加納, 英明 and Ishitsuka, Kei and Koide, Masahiro and Yoshida, Masaki and Segawa, Hiroki and Leproux, Philippe and Couderc, Vincent and Watanabe, Makoto M. and Kano, Hideaki},
 issue = {1},
 journal = {Journal of Raman Spectroscopy},
 month = {Jan},
 note = {We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis C[DOUBLE BOND]C stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy.},
 pages = {8--15},
 title = {Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source},
 volume = {48},
 year = {2017}
}