{"created":"2021-03-01T07:19:57.626537+00:00","id":40562,"links":{},"metadata":{"_buckets":{"deposit":"e9401349-4175-41ce-bfc7-0b7cf2fa1cbd"},"_deposit":{"id":"40562","owners":[],"pid":{"revision_id":0,"type":"depid","value":"40562"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00040562","sets":["2780:5486","3:62:5595:5743"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"e00038-15","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"mSphere"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"須田, 恭之"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sporulation of Saccharomyces cerevisiae is a developmental process in which an ascus containing four haploid spores forms from a diploid cell. During this process, newly formed membrane structures called prospore membranes extend along the nuclear envelope and engulf and package daughter nuclei along with cytosol and organelles to form precursors of spores. Proteins involved in prospore membrane extension, Vps13 and Spo71, have recently been reported; however, the overall mechanism of membrane extension remains unclear. Here, we identified Spo73 as an additional factor involved in prospore membrane extension. Analysis of a spo73∆ mutant revealed that it shows defects similar to those of a spo71∆ mutant during prospore membrane formation. Spo73 localizes to the prospore membrane, and this localization is independent of Spo71 and Vps13. In contrast, a Spo73 protein carrying mutations in a surface basic patch mislocalizes to the cytoplasm and overexpression of Spo71 can partially rescue localization to the prospore membrane. Similar to spo71∆ mutants, spo73∆ mutants display genetic interactions with the mutations in the SMA2 and SPO1 genes involved in prospore membrane bending. Further, our bioinformatic analysis revealed that Spo73 is a dysferlin domain-only protein. Thus, these results suggest that a dysferlin domain-only protein, Spo73, functions with a dual pleckstrin homology domain protein, Spo71, in prospore membrane extension. Analysis of Spo73 will provide insights into the conserved function of dysferlin domains, which is related to dysferlinopathy.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Microbiology"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27303688","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1128/mSphere.00038-15","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2015 Okumura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2379-5042","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okumura, Yuuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Tsuyoshi S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Takayuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inoue, Ichiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suda, Yasuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Tetsuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakanishi, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamura, Shugo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gao, Xiao-Dong"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tachikawa, Hiroyuki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-13"}],"displaytype":"detail","filename":"ｍSphere_1-1.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"ｍSphere_1-1","url":"https://tsukuba.repo.nii.ac.jp/record/40562/files/ｍSphere_1-1.pdf"},"version_id":"b5be9ac6-fc11-4a39-953a-076454157bf4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Dysferlin Domain-Only Protein, Spo73, Is Required for Prospore Membrane Extension in Saccharomyces cerevisiae","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Dysferlin Domain-Only Protein, Spo73, Is Required for Prospore Membrane Extension in Saccharomyces cerevisiae"}]},"item_type_id":"5","owner":"1","path":["5486","5743"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-03-13"},"publish_date":"2017-03-13","publish_status":"0","recid":"40562","relation_version_is_last":true,"title":["The Dysferlin Domain-Only Protein, Spo73, Is Required for Prospore Membrane Extension in Saccharomyces cerevisiae"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:11:00.091000+00:00"}