{"created":"2021-03-01T07:18:56.156336+00:00","id":39633,"links":{},"metadata":{"_buckets":{"deposit":"b604320d-0668-4c9a-b574-5c49e81482cb"},"_deposit":{"id":"39633","owners":[],"pid":{"revision_id":0,"type":"depid","value":"39633"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00039633","sets":["2780:5486","3:62:5592:3565"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"17","bibliographicPageEnd":"3261","bibliographicPageStart":"3251","bibliographicVolumeNumber":"129","bibliographic_titles":[{"bibliographic_title":"Journal of cell science"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"須田, 恭之"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Proteins synthesized in the endoplasmic reticulum (ER) are transported to the Golgi and then sorted to their destinations. For their passage through the Golgi, one widely accepted mechanism is cisternal maturation. Cisternal maturation is fulfilled by the retrograde transport of Golgi-resident proteins from later to earlier cisternae, and candidate carriers for this retrograde transport are coat protein complex I (COPI)-coated vesicles. We examined the COPI function in cisternal maturation directly by 4D observation of the transmembrane Golgi-resident proteins in living yeast cells. COPI temperature-sensitive mutants and induced degradation of COPI proteins were used to knockdown COPI function. For both methods, inactivation of COPI subunits Ret1 and Sec21 markedly impaired the transition from cis to medial and to trans cisternae. Furthermore, the movement of cisternae within the cytoplasm was severely restricted when COPI subunits were depleted. Our results demonstrate the essential roles of COPI proteins in retrograde trafficking of the Golgi-resident proteins and dynamics of the Golgi cisternae.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":" The Company of Biologists Ltd"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27445311","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":" 10.1242/jcs.193367","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016. Published by The Company of Biologists Ltd"},{"subitem_rights":"This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9533","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694823","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishii, Midori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suda, Yasuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kurokawa, Kazuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakano, Akihiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-11-07"}],"displaytype":"detail","filename":"JCS_129-17-3251.pdf","filesize":[{"value":"3.2 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"JCS_129-17-3251","url":"https://tsukuba.repo.nii.ac.jp/record/39633/files/JCS_129-17-3251.pdf"},"version_id":"05bacad6-37a6-4fb0-99de-6ca560fae8ff"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"COPI is essential for Golgi cisternal maturation and dynamics","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"COPI is essential for Golgi cisternal maturation and dynamics"}]},"item_type_id":"5","owner":"1","path":["5486","3565"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-11-07"},"publish_date":"2016-11-07","publish_status":"0","recid":"39633","relation_version_is_last":true,"title":["COPI is essential for Golgi cisternal maturation and dynamics"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:09:48.037632+00:00"}