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Analysis of the aplyronine A-induced protein-protein interaction between actin and tubulin by surface plasmon resonance
http://hdl.handle.net/2241/00143388
http://hdl.handle.net/2241/00143388f6ecba84-6d0c-463f-b6bd-799573169cf2
名前 / ファイル | ライセンス | アクション |
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BMC_24-12 (747.2 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2016-07-26 | |||||
タイトル | ||||||
タイトル | Analysis of the aplyronine A-induced protein-protein interaction between actin and tubulin by surface plasmon resonance | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Hirayama, Yuichiro
× Hirayama, Yuichiro× Yamagishi, Kota× Suzuki, Tomohiro× Kawagishi, Hirokazu× Kita, Masaki× 木越, 英夫 |
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著者別名 |
木越, 英夫
× 木越, 英夫× 北, 将樹 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The antitumor macrolide aplyronine A induces protein–protein interaction (PPI) between actin and tubu-lin to exert highly potent biological activities. The interactions and binding kinetics of these molecules were analyzed by the surface plasmon resonance with biotinylated aplyronines or tubulin as ligands. Strong binding was observed for tubulin and actin with immobilized aplyronine A. These PPIs were almost completely inhibited by one equivalent of either aplyronine A or C, or mycalolide B. In contrast, a non-competitive actin-depolymerizing agent, latrunculin A, highly accelerated their association. Significant binding was also observed for immobilized tubulin with an actin–aplyronine A complex, and the dissociation constant K D was 1.84 l M. Our method could be used for the quantitative analysis of the PPIs between two polymerizing proteins stabilized with small agents. |
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書誌情報 |
Bioorganic & medicinal chemistry 巻 24, 号 12, p. 2809-2814, 発行日 2016-06 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0968-0896 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10938083 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 27161875 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.bmc.2016.04.049 | |||||
権利 | ||||||
権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier |