@article{oai:tsukuba.repo.nii.ac.jp:00038245,
 author = {Baba, Osamu and Hasegawa, Shogo and Nagai, Hiroki and 内田, 文彦 and UCHIDA, Fumihiko and Yamatoji, Masanobu and 菅野, 直美 and KANNO, Naomi and 山縣, 憲司 and YAMAGATA, Kenji and 酒井, 俊 and SAKAI, Satoshi and 柳川, 徹 and YANAGAWA, Toru and 武川, 寛樹 and BUKAWA, Hiroki},
 issue = {4},
 journal = {Journal of oral pathology & medicine},
 month = {Apr},
 note = {Background<br/>Abnormal miRNA expression was recently implicated in the metastasis of oral squamous cell carcinoma (OSCC) and with a poor prognosis. The initiation of the invasion-metastasis cascade involves epithelial-mesenchymal transition (EMT). Our aim was to clarify how miRNA, especially miR-155-5p misexpression contributes to OSCC metastasis through EMT.<br/><br/>Methods<br/>We collected tumor samples from 73 subjects with OSCC. The samples were analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and correlations between miR-155-5p levels and clinical characteristics were investigated. OSCC cell lines were analyzed by miRNA microarray and by transfection with a miR-155-5p mimic or inhibitor, followed by proliferation and wound-healing migration assays. qRT-PCR analyses of EMT makers in cells transfected with miR-155-5p inhibitor were performed.<br/><br/>Results<br/>We found high miR-155-5p expression in tissue samples from subjects with OSCC that had metastasized to cervical lymph nodes. HSC-3 cells also strongly expressed miR-155-5p. The epithelial marker E-cadherin was strongly expressed in HSC-3 cells transfected with miR-155-5p inhibitor, and we observed elevated SOCS1 and decreased STAT3 expression in these cells.<br/><br/>Conclusions<br/>Our results suggest that miR-155-5p causes OSCC to metastasize, and could serve as a novel therapeutic target for OSCC.},
 pages = {248--255},
 title = {MicroRNA-155-5p is associated with oral squamous cell carcinoma metastasis and poor prognosis},
 volume = {45},
 year = {2016},
 yomi = {ウチダ, フミヒコ and カンノ, ナオミ and ヤマガタ, ケンジ and サカイ, サトシ and ヤナガワ, トオル and ブカワ, ヒロキ}
}