{"_buckets": {"deposit": "ce6a4e14-d234-4666-abed-89c236f57245"}, "_deposit": {"id": "36664", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "36664"}, "status": "published"}, "_oai": {"id": "oai:tsukuba.repo.nii.ac.jp:00036664"}, "item_5_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-12", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "17853", "bibliographicVolumeNumber": "5", "bibliographic_titles": [{"bibliographic_title": "Scientific reports"}]}]}, "item_5_creator_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5317, \u5c06\u6a39"}], "nameIdentifiers": [{"nameIdentifier": "643", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u6728\u8d8a, \u82f1\u592b"}], "nameIdentifiers": [{"nameIdentifier": "88", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90169839", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000090169839"}, {"nameIdentifier": "0000000631", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000000631"}]}]}, "item_5_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Pyrene-conjugated compounds are detected by label-assisted laser desorption/ionization mass spectrometry (LA-LDI MS) without matrixes. We found that 6-amidopyrene derivatives were highly detectable by the LDI MS instrument equipped with a 355\u2009nm laser. In a certain case of a 6-amidopyrene derivative, a molecular ion peak [M]+\u2022 and a characteristic fragment ion peak [M\u201342]+\u2022 were detected in an amount of only 10\u2009fmol. The latter peak, corresponding to the 6-aminopyrene fragment, might be generated in situ by the removal of ketene (CH2=C=O) from the parent molecule. A photoaffinity amidopyrene derivative of an antitumor macrolide aplyronine A (ApA\u2013PaP) was synthesized, which showed potent cytotoxicity and actin-depolymerizing activity. In an LDI MS analysis of the MeOH- and water-adducts of ApA\u2013PaP, oxime N\u2013O bonds as well as amidopyrene N-acetyl moieties were preferentially cleaved, and their internal structures were confirmed by MS/MS analysis. Amidopyrene moiety might enhance fragmentation and stabilize the cleaved fragments by intramolecular or intermolecular weak interactions including hydrogen bonding. Our chemical probe methods might contribute to a detailed analysis of binding modes between various ligands and target biomacromolecules that include multiple and weak interactions.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_27": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nature Publishing Group"}]}, "item_5_relation_10": {"attribute_name": "PubMed\u756a\u53f7", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "26667050", "subitem_relation_type_select": "PMID"}}]}, "item_5_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1038/srep17853", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article\u2019s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/"}]}, "item_5_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2045-2322", "subitem_source_identifier_type": "ISSN"}]}, "item_5_subject_20": {"attribute_name": "NII\u30b5\u30d6\u30b8\u30a7\u30af\u30c8", "attribute_value_mlt": [{"subitem_subject": "\u5316\u5b66", "subitem_subject_scheme": "Other"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yoneda, Kozo"}], "nameIdentifiers": [{"nameIdentifier": "124903", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hu, Yaping "}], "nameIdentifiers": [{"nameIdentifier": "124904", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kita, Masaki"}], "nameIdentifiers": [{"nameIdentifier": "124905", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "\u6728\u8d8a, \u82f1\u592b"}, {"creatorName": "\u30ad\u30b4\u30b7, \u30d2\u30c7\u30aa", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "88", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90169839", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://nrid.nii.ac.jp/ja/nrid/1000090169839"}, {"nameIdentifier": "0000000631", "nameIdentifierScheme": "\u7b51\u6ce2\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7", "nameIdentifierURI": "http://trios.tsukuba.ac.jp/researcher/0000000631"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-01-21"}], "displaytype": "detail", "download_preview_message": "", "filename": "SR-5_17853.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 11000000, "url": {"label": "SR-5_17853", "url": "https://tsukuba.repo.nii.ac.jp/record/36664/files/SR-5_17853.pdf"}, "version_id": "7f3e371c-acd3-437d-814a-b0824e9d48b8"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "6-Amidopyrene as a label-assisted laser desorption/ionization (LA-LDI) enhancing tag: development of photoaffinity pyrene derivative", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "6-Amidopyrene as a label-assisted laser desorption/ionization (LA-LDI) enhancing tag: development of photoaffinity pyrene derivative"}]}, "item_type_id": "5", "owner": "1", "path": ["29/297", "117/1443", "3/62/5601/1884"], "permalink_uri": "http://hdl.handle.net/2241/00133823", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2016-01-21"}, "publish_date": "2016-01-21", "publish_status": "0", "recid": "36664", "relation": {}, "relation_version_is_last": true, "title": ["6-Amidopyrene as a label-assisted laser desorption/ionization (LA-LDI) enhancing tag: development of photoaffinity pyrene derivative"], "weko_shared_id": 5}