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Application of Biomaterials and Inkjet Printing to Develop Bacterial Culture System
http://hdl.handle.net/2241/00126843
http://hdl.handle.net/2241/00126843ec95ba3c-5ce2-4002-bf50-12e16e0e4360
名前 / ファイル | ライセンス | アクション |
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AMSE_2015-290790 (3.9 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2015-09-24 | |||||
タイトル | ||||||
タイトル | Application of Biomaterials and Inkjet Printing to Develop Bacterial Culture System | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Srimongkon, Tithimanan
× Srimongkon, Tithimanan× Mandai, Shusaku× Enomae, Toshiharu |
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著者別名 |
江前, 敏晴
× 江前, 敏晴 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We created an automated bioassay system based on inkjet printing. Compared to conventional manual bacterial culture systems our printing approach improves the quality as well as the processing speed. A hydrophobic/hydrophilic pattern as a container supporting a culture medium was built on filter paper using a toluene solution of polystyrene for hydrophobization, followed by toluene printing to create several hydrophilic areas. As culture media we used a novel poly(vinyl alcohol) based hydrogel and a standard calcium alginate hydrogel. The poly(vinyl alcohol) hydrogel was formed by physical crosslinking poly(vinyl alcohol) with adipic acid dihydrazide solutions. The conditions of poly(vinyl alcohol) gelation were optimized for inkjet printability and the optimum mixture ratio was determined. The calcium alginate hydrogel was formed by chemical reaction between sodium alginate and CaCl2 solutions. Together with nutrients both hydrogel solutions were successfully printed on paper by means of the modified inkjet printer. The amount of each solution was demanded simply by outputting CMYK values. In the last step bacterial cells were printed on both hydrogel media. For both media we achieved a stable bacteria growth which was confirmed by microscopical imaging of the developed bacterial colonies. | |||||
書誌情報 |
Advances in materials science and engineering 巻 2015, p. 290790, 発行日 2015 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1687-8434 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1155/2015/290790 | |||||
権利 | ||||||
権利情報 | © 2015 Tithimanan Srimongkon et al.This is an open access article distributed under theCreativeCommonsAttribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the originalwork is properly cited. | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Hindawi Publishing Corporation |