@article{oai:tsukuba.repo.nii.ac.jp:00033593,
 author = {竹内, 薫 and Ohkura, Takashi and Minakuchi, Moeko and Sagai, Mami and Kokuho, Takehiro and Konishi, Misako and Kameyama, Ken-ichiro and Takeuchi, Kaoru},
 journal = {Virology},
 month = {Feb},
 note = {Bovine parainfluenza virus type 3 (BPIV3) is an important pathogen associated with bovine respiratory disease complex (BRDC). We have generated a recombinant BPIV3 expressing enhanced green fluorescent protein (rBPIV3-EGFP) based on the BN-1 strain isolated in Japan. After intranasal infection of hamsters with rBPIV3-EGFP, EGFP fluorescence was detected in the upper respiratory tract including the nasal turbinates, pharynx, larynx, and trachea. In the nasal turbinates, rBPIV3-EGFP attained high titers (>106 TCID50/g of tissue) 2–4 days after infection. Ciliated epithelial cells in the nasal turbinates and trachea were infected with rBPIV3-EGFP. Histopathological analysis indicated that mucosal epithelial cells in bronchi were shed by 6 days after infection, leaving non-ciliated cells, which may have increased susceptibility to bacterial infection leading to the development of BRDC. These data indicate that rBPIV3-EGFP infection of hamsters is a useful small animal model for studying the development of BPIV3-associated BRDC.},
 pages = {134--140},
 title = {Infection of the upper respiratory tract of hamsters by the bovine parainfluenza virus type 3 BN-1 strain expressing enhanced green fluorescent protein},
 volume = {476},
 year = {2015}
}