{"created":"2021-03-01T07:12:00.525045+00:00","id":33247,"links":{},"metadata":{"_buckets":{"deposit":"189f26b6-4262-4196-b510-dc23948e0736"},"_deposit":{"id":"33247","owners":[],"pid":{"revision_id":0,"type":"depid","value":"33247"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00033247","sets":["117:306","3:62:5592:3017"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"592","bibliographicPageStart":"587","bibliographicVolumeNumber":"104","bibliographic_titles":[{"bibliographic_title":"Journal of pharmaceutical sciences"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"白木, 賢太郎"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Noncovalent PEGylation has great potential for stabilization of therapeutic proteins. Here, we demonstrated that the noncovalent PEGylation with a PEGylated polyelectrolyte stabilized a therapeutic protein, l-asparaginase (ASNase). Anionic ASNase and cationic poly(ethylene glycol)-block-poly(N,N-dimethylaminoethyl methacrylate) (PEG-b-PAMA) formed a water-soluble protein–polyelectrolyte complex (PPC) without loss of secondary structure and enzyme activity. PPC with PEG-b-PAMA successfully inhibited the shaking-induced inactivation and aggregation of ASNase as well as protease digestion, corresponding to the behaviors of covalently PEGylated ASNase. Thus, noncovalent PEGylation by PEGylated polyelectrolytes is a new candidate for handling of therapeutic proteins.","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Pharmaceutical Association"},{"subitem_publisher":"Wiley"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"25354692","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/jps.24217","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2014 Wiley Periodicals, Inc. and the American Pharmacists Association"},{"subitem_rights":"This is tthe peer reviewed version of the following article:  J. Pharm. Sci., 104: 587–592. doi: 10.1002/jps.24217, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jps.24217/epdf. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-3549","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"1520-6017","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00704450","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kurinomaru, Takaaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiraki, Kentaro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-03-01"}],"displaytype":"detail","filename":"JPS_104-2.pdf","filesize":[{"value":"732.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JPS_104-2","url":"https://tsukuba.repo.nii.ac.jp/record/33247/files/JPS_104-2.pdf"},"version_id":"8ad7a2e6-86b7-40b7-b8f1-c513e2958acf"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte"}]},"item_type_id":"5","owner":"1","path":["306","3017"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-04-06"},"publish_date":"2015-04-06","publish_status":"0","recid":"33247","relation_version_is_last":true,"title":["Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T10:12:59.378502+00:00"}