@article{oai:tsukuba.repo.nii.ac.jp:00033247,
 author = {白木, 賢太郎 and Kurinomaru, Takaaki and Shiraki, Kentaro},
 issue = {2},
 journal = {Journal of pharmaceutical sciences},
 month = {Feb},
 note = {Noncovalent PEGylation has great potential for stabilization of therapeutic proteins. Here, we demonstrated that the noncovalent PEGylation with a PEGylated polyelectrolyte stabilized a therapeutic protein, l-asparaginase (ASNase). Anionic ASNase and cationic poly(ethylene glycol)-block-poly(N,N-dimethylaminoethyl methacrylate) (PEG-b-PAMA) formed a water-soluble protein–polyelectrolyte complex (PPC) without loss of secondary structure and enzyme activity. PPC with PEG-b-PAMA successfully inhibited the shaking-induced inactivation and aggregation of ASNase as well as protease digestion, corresponding to the behaviors of covalently PEGylated ASNase. Thus, noncovalent PEGylation by PEGylated polyelectrolytes is a new candidate for handling of therapeutic proteins.},
 pages = {587--592},
 title = {Noncovalent PEGylation of l-Asparaginase Using PEGylated Polyelectrolyte},
 volume = {104},
 year = {2015}
}