{"created":"2021-03-01T07:10:23.316878+00:00","id":31720,"links":{},"metadata":{"_buckets":{"deposit":"f1677c30-f9e6-4ac4-94cc-ea664f051d33"},"_deposit":{"id":"31720","owners":[],"pid":{"revision_id":0,"type":"depid","value":"31720"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00031720","sets":["160:855","160:857","3:62:5297:376"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1458","bibliographicPageStart":"1451","bibliographicVolumeNumber":"1841","bibliographic_titles":[{"bibliographic_title":"Biochimica et biophysica acta. Molecular and cell biology of lipids"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"白岩, 善博"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 石根"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The coccolithophorid Emiliania huxleyi is a bloom-forming marine phytoplankton thought to play a key role as a biological pump that transfers carbon from the surface to the bottom of the ocean, thus contributing to the global carbon cycle. This alga is also known to accumulate a variety of polyunsaturated fatty acids. At 25 °C, E. huxleyi produces mainly 14:0, 18:4n − 3, 18:5n − 3 and 22:6n − 3. When the cells were transferred from 25 °C to 15 °C, the amount of unsaturated fatty acids, i.e. 18:1n − 9, 18:3n − 3 and 18:5n − 3, gradually increased. Among the predicted desaturase genes whose expression levels were up-regulated at low temperature, we identified a gene encoding novel ∆15 fatty acid desaturase, EhDES15, involved in the production of n − 3 polyunsaturated fatty acids in E. huxleyi. This desaturase contains a putative transit sequence for localization in chloroplasts and a ∆6 desaturase-like domain, but it does not contain a cytochrome b5 domain nor typical His-boxes found in ∆15 desaturases. Heterologous expression of EhDES15 cDNA in cyanobacterium Synechocystis sp. PCC 6803 cells increased the level of n − 3 fatty acid species, which are produced at low levels in wild-type cells grown at 30 °C. The orthologous genes are only conserved in the genomes of prasinophytes and cryptophytes. The His-boxes conserved in orthologues varied from that of the canonical ∆15 desaturases. These results suggested the gene encodes a novel ∆15 desaturase responsible for the synthesis of 18:3n − 3 from 18:2n − 6 in E. huxleyi.","subitem_description_type":"Other"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ELSEVIER"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"25046625","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.bbalip.2014.07.010","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2014 Elsevier B.V."},{"subitem_rights":"“NOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et biophysica acta. Molecular and cell biology of lipids. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et biophysica acta. Molecular and cell biology of lipids,1841,10,2014. doi:10.1016/j.bbalip.2014.07.010¨"}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1388-1981","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11300409","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kotajima, Tomonori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shiraiwa, Yoshihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Iwane"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2014-11-25"}],"displaytype":"detail","filename":"BBA_1841-10.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"BBA_1841-10","url":"https://tsukuba.repo.nii.ac.jp/record/31720/files/BBA_1841-10.pdf"},"version_id":"df8d7501-1a18-4ac3-b383-c7eae58344d7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Functional screening of a novel Δ15 fatty acid desaturase from the coccolithophorid Emiliania huxleyi","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Functional screening of a novel Δ15 fatty acid desaturase from the coccolithophorid Emiliania huxleyi"}]},"item_type_id":"5","owner":"1","path":["855","857","376"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-11-21"},"publish_date":"2014-11-21","publish_status":"0","recid":"31720","relation_version_is_last":true,"title":["Functional screening of a novel Δ15 fatty acid desaturase from the coccolithophorid Emiliania huxleyi"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:00:00.485122+00:00"}