@article{oai:tsukuba.repo.nii.ac.jp:00030924,
 author = {松野, 幸也 and 藤村, 達人 and Matsuno, Koya and Fujimura, Tatsuhito},
 journal = {Plant Science},
 month = {Mar},
 note = {A pathway of phytic acid (PA) synthesis in plants has been revealed via investigations of low phytic acid mutants. However, the regulation of this pathway is not well understood because it is difficult to control the environments of cells in the seeds, where PA is mainly synthesized. We modified a rice suspension culture system in order to study the regulation of PA synthesis. Rice cells cultured with abscisic acid (ABA) accumulate PA at higher levels than cells cultured without ABA, and PA accumulation levels increase with ABA concentration. On the other hand, higher concentrations of sucrose or inorganic phosphorus do not affect PA accumulation. Mutations in the genes RINO1, OsMIK, OsIPK1 and OsLPA1 have each been reported to confer low phytic acid phenotypes in seeds. Each of these genes is upregulated in cells cultured with ABA. OsITPK4 and OsITPK6 are upregulated in cells cultured with ABA and in developing seeds. These results suggest that the regulation of PA synthesis is similar between developing seeds and cells in this suspension culture system. This system will be a powerful tool for elucidating the regulation of PA synthesis.},
 pages = {152--157},
 title = {Induction of phytic acid synthesis by abscisic acid in suspension-cultured cells of rice},
 volume = {217–218},
 year = {2014}
}