@article{oai:tsukuba.repo.nii.ac.jp:00029531,
 author = {出村, 幹英 and 渡邉, 信 and Demura, Mikihide and Ioki, Motohide and Kawachi, Masanobu and Nakajima, Nobuyoshi and Watanabe, Makoto M.},
 issue = {1},
 journal = {Journal of applied phycology},
 month = {Feb},
 note = {Botryococcus braunii Kützing, a green colonial microalga, occurs worldwide in both freshwater and brackish water environments. Despite considerable attention to B. braunii as a potential source of renewable fuel, many ecophysiological properties of this alga remain unknown. Here, we examined the desiccation and temperature tolerances of B. braunii using two newly isolated strains BOD-NG17 and BOD-GJ2. Both strains survived through 6- and 8-month desiccation treatments but not through a 12-month treatment. Interestingly, the desiccation-treated cells of B. braunii gained tolerance to extreme temperature shifts, i.e., high temperature (40 °C) and freezing (−20 °C). Both strains survived for at least 4 and 10 days at 40 and −20 °C, respectively, while the untreated cells barely survived at these temperatures. These traits would enable long-distance dispersal of B. braunii cells and may account for the worldwide distribution of this algal species. Extracellular substances such as polysaccharides and hydrocarbons seem to confer the desiccation tolerance.},
 pages = {49--53},
 title = {Desiccation tolerance of Botryococcus braunii (Trebouxiophyceae, Chlorophyta) and extreme temperature tolerance of dehydrated cells},
 volume = {26},
 year = {2014}
}