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Novel promoters that induce specific transgene expression during the green to ripening stages of tomato fruit development
http://hdl.handle.net/2241/119659
http://hdl.handle.net/2241/119659e795ecb9-c9db-4e19-9baa-6dd4d76a6b68
名前 / ファイル | ライセンス | アクション |
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PCR_31-8.pdf (630.5 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2013-09-06 | |||||
タイトル | ||||||
タイトル | Novel promoters that induce specific transgene expression during the green to ripening stages of tomato fruit development | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Hiwasa-Tanase, Kyoko
× Hiwasa-Tanase, Kyoko× Kuroda, Hirofumi× Hirai, Tadayoshi× Aoki, Koh× Takane, Kenichi× Ezura, Hiroshi |
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著者別名 |
江面, 浩
× 江面, 浩 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Fruit-specific promoters have been used as genetic engineering tools for studies on molecular mechanism of fruit development and advance in fruit quality and additional value by increasing functional component. Especially fruit-ripening specific promoters have been well utilized and studied in tomato; however, few studies have reported the development of promoters that act at fruit developing stages such as immature green and mature green periods. In this study, we report novel promoters for gene expression during the green to ripening stages of tomato fruit development. Genes specifically expressed at tomato fruit were selected using microarray data. Subsequent to confirmation of the expression of the selected 12 genes, upstream DNA fragments of the genes LA22CD07, Les.3122.2.A1_a_at and LesAffx.6852.1.S1_at which specifically expressed at fruit were isolated from tomato genomic DNA as promoter regions. Isolated promoter regions were fused with the GUS gene and the resultant constructs were introduced into tomato by agrobacterium-mediated transformation for evaluation of promoter activity in tomato fruit. The two promoters of LA22CD07, and LesAffx.6852.1.S1_at showed strong activity in the fruit, weak activity in the flower and undetectable activity in other tissues. Unlike well-known fruit-ripening specific promoters, such as the E8 promoter, these promoters exhibited strong activity in green fruit in addition to red-ripening fruit, indicating that the promoters are suitable for transgene expression during green to ripening stages of tomato fruit development. | |||||
書誌情報 |
Plant cell reports 巻 31, 号 8, p. 1415-1424, 発行日 2012-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0721-7714 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10635864 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 22481231 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s00299-012-1257-5 | |||||
権利 | ||||||
権利情報 | © Springer-Verlag 2012 The original publication is available at www.springerlink.com | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer-Verlag | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/119659 | |||||
識別子タイプ | HDL |