@article{oai:tsukuba.repo.nii.ac.jp:00029065,
 author = {坂本, 和一 and Kim, Hyojung and Sakamoto, Kazuichi},
 issue = {2},
 journal = {Cell biology international},
 month = {Feb},
 note = {EGCG [(−)-epigallocatechin gallate], tea catechin, is one of the compounds that has been reported to act against obesity and diabetes. To determine the effect of EGCG on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different catechins. Oil Red O staining showed significantly reduced intracellular lipid accumulation, especially with EGCG. Cell cycle analysis showed that EGCG inhibited cell proliferation by disturbing the cell cycle during the clonal expansion of 3T3-L1. RT-PCR (real-time PCR) demonstrated that EGCG noticeably reduced mRNA expression of PPARγ (peroxisome proliferator-activated receptor γ), C/EBPα (CCAAT/enhancer-binding protein α) and FoxO1 (forkhead box class O1). EGCG also caused a significant decrease in the transcription of FoxO1 – the forkhead transcription factor class O1 involved in adipocyte differentiation – via the PI3K (phosphoinositide 3-kinase)/Akt and MEK [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase] pathways. These results suggest that EGCG suppresses the clonal expansion of adipocytes by inactivating FoxO1 via insulin signalling and stress-dependent MAPK pathways.},
 pages = {147--153},
 title = {(−)-Epigallocatechin gallate suppresses adipocyte differentiation through the MEK/ERK and PI3K/Akt pathways},
 volume = {36},
 year = {2012}
}