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Assessment of wastewater-irrigated soil containing heavy metals and establishment of specific biomarkers
http://hdl.handle.net/2241/117711
http://hdl.handle.net/2241/1177113e34f548-105a-4632-9160-143873a7bc52
名前 / ファイル | ライセンス | アクション |
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EES_84-54-62.pdf (669.9 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2012-10-26 | |||||
タイトル | ||||||
タイトル | Assessment of wastewater-irrigated soil containing heavy metals and establishment of specific biomarkers | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Fredj, F. Ben
× Fredj, F. Ben× Han, J.× Irie, M.× Funamizu, N.× Ghrabi, A.× Isoda, H. |
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著者別名 |
入江, 光輝
× 入江, 光輝× 礒田, 博子 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Irrigation with treated wastewater (TWW) is a vital alternative for arid and semi-arid lands but it poses pollution-risk to soil, vegetation and groundwater. Therefore, in the present study, in vitro bioassays were used to evaluate the adverse effects of TWW and irrigated-soil extract sample, on mammalian cells, with respect to heavy metal—Ni, Cd, Pb, Fe, Al-content. The heat shock protein (HSP) 47, E-screen, and transepithelial electrical resistance (TEER) assays served to investigate the stress response of treated-HSP47-transfected Chinese hamster ovary (CHO) cells, the estrogenic activity of the samples in MCF-7 breast cancer cells, and the barrier function (BF) of Caco-2 cells. Furthermore, proteomics analyses were performed to shed light on involved mechanisms and to establish pollution biomarkers. Results showed that the TWW elicited a stress response on HSP cells from 0.1% concentration while soil extract samples exhibited a stress at 1%. TWW induced an estrogenic activity at 10%; up-regulating cell proliferation and tumor-related proteins. Soil extract triggered the enhanced expression of HSP70 family proteins as survival mechanisms against their cytotoxicity toward MCF-7 cells. Moreover, depending on the concentration, 1% of soil extract from 20 cm depth (T20) resulted in a disruption of BF in Caco-2 cells involving cell metabolism, protein synthesis and tumor marker proteins, whereas, 5% of T20 induced the expression of BF-related proteins associated to heat shock, oxidative stress, cell proliferation and glycolytic metabolic pathway. These biological techniques were found to be extremely useful to evaluate the impact of wastewater reuse and to establish specific biomarkers that are common proteins for humans, other mammals and plants. Future studies should focus on exposure quantifications. | |||||
書誌情報 |
Ecotoxicology and environmental safety 巻 84, p. 54-62, 発行日 2012-10 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0147-6513 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00179452 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 22795889 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.ecoenv.2012.06.020 | |||||
権利 | ||||||
権利情報 | © 2012 Elsevier Inc.NOTICE: this is the author's version of a work that was accepted for publication in Ecotoxicology and Environmental Safety. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Ecotoxicology and Environmental Safety, Vol.84, Pages:54-62. doi:10.1016/j.ecoenv.2012.06.020 | |||||
著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/117711 | |||||
識別子タイプ | HDL |