@article{oai:tsukuba.repo.nii.ac.jp:00027505,
 author = {吉田, 康子 and 大澤, 良 and Yoshida, Yasuko and Ueno, Saneyoshi and Honjo, Masanori and Kitamoto, Naoko and Nagai, Mihoko and Washitani, Izumi and Tsumura, Yoshihiko and Yasui, Yasuo and Ohsawa, Ryo},
 issue = {1},
 journal = {Annals of botany},
 month = {Jul},
 note = {Background and Aims Primula sieboldii is a perennial clonal herb that is distributed around the Sea of Japan and is endangered in Japan. Its breeding system is characterized by heteromorphic self-incompatibility, and the morph ratio within a population is very important for reproductive success. The aims of this study were to construct a linkage map, map the S locus as a qualitative trait and quantitative trait loci (QTLs) for floral morphological traits related to heterostyly, and predict the morph type in wild populations by using molecular markers for devising a conservation strategy.

Methods A linkage map was constructed with 126 markers. The QTLs for four floral traits and the S locus were mapped. Using the genotypes of loci that were located near both the S locus and the QTLs with large effects, morphs of 59 wild genets were predicted.

Key Results The linkage map consisted of 14 linkage groups (LGs). The S locus was mapped to LG 7. Major QTLs for stigma and anther heights were detected in the same region as the S locus. These QTLs exhibited high logarithm of the odds scores and explained a high percentage of the phenotypic variance (>85 %). By analysing these two traits within each morph, additional QTLs for each trait were detected. Using the four loci linked to the S locus, the morphs of 43 genets in three wild populations could be predicted.

Conclusions This is the first report of a linkage map and QTL analysis for floral morphology related to heterostyly in P. sieboldii. Floral morphologies related to heterostyly are controlled by the S locus in LG 7 and by several QTLs in other LGs. Additionally, this study showed that molecular markers are effective tools for investigating morph ratios in a population containing the non-flowering individuals or during the non-flowering seasons.},
 pages = {133--142},
 title = {QTL analysis of heterostyly in Primula sieboldii and its application for morph identification in wild populations},
 volume = {108},
 year = {2011}
}