@article{oai:tsukuba.repo.nii.ac.jp:00027085,
 author = {本多, 伸一郎 and 小田, ちぐさ and 田原, 聡子 and 渋谷, 和子 and 渋谷, 彰 and Usui, Kenta and Honda, Shin-ichiro and Yoshizawa, Yuichi and Nakahashi-Oda, Chigusa and Tahara-Hanaoka, Satoko and Shibuya, Kazuko and Shibuya, Akira},
 issue = {3},
 journal = {Molecular immunology},
 month = {Mar},
 note = {Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naïve mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for ∼0.2% of the spleen cells of naïve mice, were CD45−, FDC-M2+, and ICAM-1+, and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation.},
 pages = {172--176},
 title = {Isolation and characterization of naïve follicular dendritic cells},
 volume = {50},
 year = {2012}
}