@article{oai:tsukuba.repo.nii.ac.jp:00025848,
 author = {江面, 浩 and Hirai, Tadayoshi and Shohael, Abdullah Mohammad and Kim, You-Wang and Yano, Megumu and Ezura, Hiroshi},
 issue = {12},
 journal = {Plant cell reports},
 month = {Dec},
 note = {Lettuce is a commercially important leafy
vegetable that is cultivated worldwide, and it is also a
target crop for plant factories. In this study, lettuce was
selected as an alternative platform for recombinant miraculin
production because of its fast growth, agronomic
value, and wide availability. The taste-modifying protein
miraculin is a glycoprotein extracted from the red berries of
the West African native shrub Richadella dulcifica.
Because of its limited natural availability, many attempts
have been made to produce this protein in suitable alternative
hosts. We produced transgenic lettuce with miraculin
gene driven either by the ubiquitin promoter/
terminator cassette from lettuce or a 35S promoter/nos
terminator cassette. Miraculin gene expression and miraculin
accumulation in both cassettes were compared by
quantitative real-time PCR analysis, Western blotting, and
enzyme-linked immunosorbent assay. The expression level
of the miraculin gene and protein in transgenic lettuce was
higher and more genetically stable in the ubiquitin promoter/
terminator cassette than in the 35S promoter/nos
terminator cassette. These results demonstrated that the
ubiquitin promoter/terminator cassette is an efficient platform
for the genetically stable expression of the miraculin
protein in lettuce and hence this platform is of benefit for
recombinant miraculin production on a commercial scale.},
 pages = {2255--2265},
 title = {Ubiquitin promoter–terminator cassette promotes genetically stable expression of the taste-modifying protein miraculin in transgenic lettuce},
 volume = {30},
 year = {2011}
}