{"created":"2021-03-01T07:03:18.844990+00:00","id":25210,"links":{},"metadata":{"_buckets":{"deposit":"929a6454-0951-4a1e-9774-531a21ef6f22"},"_deposit":{"id":"25210","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25210"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00025210","sets":["2780:1287","2780:926","3:62:5592:1617"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-08","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"62","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Journal of biomedical science"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鶴嶋, 英夫"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"松村, 明"}],"nameIdentifiers":[{},{},{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background\n\nSafe and efficient gene transfer systems are needed for tissue engineering. We have developed an apatite composite layer including the bone morphogenetic protein-2 (BMP-2) gene and fibronectin (FB), and we evaluated its ability to induce bone formation.\nMethods\n\nAn apatite composite layer was evaluated to determine the efficiency of gene transfer to cells cultured on it. Cells were cultured on a composite layer including the BMP-2 gene and FB, and BMP-2 gene expression, BMP-2 protein concentrations, alkaline phosphatase (ALP) activity, and osteocalcin (OC) concentrations were measured. A bone defect on the cranium of rats was treated with hydroxyapatite (HAP)-coated ceramic buttons with the apatite composite layer including the BMP-2 gene and FB (HAP-BMP-FB). The tissue concentration of BMP-2, bone formation, and the expression levels of the BMP-2, ALP, and OC genes were all quantified.\nResults\n\nThe apatite composite layer provided more efficient gene transfer for the cultured cells than an apatite composite layer without FB. The BMP-2 concentration was approximately 100~600 pg/mL in the cell-culture medium. Culturing the cells on the apatite composite layer for 27 days increased ALP activity and OC concentrations. In animal experiments, the tissue concentrations of BMP-2 were over 100 pg/mg in the HAP-BMP-FB group and approximately 50 pg/mg in the control groups. Eight weeks later, bone formation was more enhanced in the HAP-BMP-FB group than in the control groups. In the tissues surrounding the HAP button, the gene expression levels of ALP and OC increased.\nConclusion\n\nThe BMP-2 gene-FB-apatite composite layer might be useful for bone engineering.","subitem_description_type":"Abstract"}]},"item_5_identifier_34":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2241/114083"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central Ltd."}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"21859498","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1186/1423-0127-18-62","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 Zhang et al; licensee BioMed Central Ltd.  This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"10217770","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10955606","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zhang, Wei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsurushima, Hideo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oyane, Ayako"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yazaki, Yushin"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sogo, Yu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Atsuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsumura, Akira"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2013-12-25"}],"displaytype":"detail","filename":"JBS_18-1.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"JBS_18-1.pdf","url":"https://tsukuba.repo.nii.ac.jp/record/25210/files/JBS_18-1.pdf"},"version_id":"c28a8c9e-9b05-4cf6-8229-8ecc66680fca"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"BMP-2 gene-fibronectin-apatite composite layer enhances bone formation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"BMP-2 gene-fibronectin-apatite composite layer enhances bone formation"}]},"item_type_id":"5","owner":"1","path":["1287","926","1617"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-10-13"},"publish_date":"2011-10-13","publish_status":"0","recid":"25210","relation_version_is_last":true,"title":["BMP-2 gene-fibronectin-apatite composite layer enhances bone formation"],"weko_creator_id":"1","weko_shared_id":5},"updated":"2022-04-27T09:53:50.845183+00:00"}