{"created":"2021-03-01T07:03:18.443643+00:00","id":25204,"links":{},"metadata":{"_buckets":{"deposit":"2b976e91-31f4-48b9-9f11-57f06f0baae3"},"_deposit":{"id":"25204","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25204"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:00025204","sets":["2871:2873:808","3:62:5297:569"]},"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"30","bibliographicPageEnd":"7490","bibliographicPageStart":"7479","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Biomaterials"}]}]},"item_5_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福田, 淳二"}],"nameIdentifiers":[{}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A major challenge in tissue engineering is to reproduce the native 3D microvascular architecture fundamental for in vivo functions. Current approaches still lack a network of perfusable vessels with native 3D structural organization. Here we present a new method combining self-assembled monolayer (SAM)-based cell transfer and gelatin methacrylate hydrogel photopatterning techniques for microengineering vascular structures. Human umbilical vein cell (HUVEC) transfer from oligopeptide SAM-coated surfaces to the hydrogel revealed two SAM desorption mechanisms: photoinduced and electrochemically triggered. The former, occurs concomitantly to hydrogel photocrosslinking, and resulted in efficient (>97%) monolayer transfer. The latter, prompted by additional potential application, preserved cell morphology and maintained high transfer efficiency of VE-cadherin positive monolayers over longer culture periods. This approach was also applied to transfer HUVECs to 3D geometrically defined vascular-like structures in hydrogels, which were then maintained in perfusion culture for 15 days. As a step toward more complex constructs, a cell-laden hydrogel layer was photopatterned around the endothelialized channel to mimic the vascular smooth muscle structure of distal arterioles. This study shows that the coupling of the SAM-based cell transfer and hydrogel photocrosslinking could potentially open up new avenues in engineering more complex, vascularized tissue constructs for regenerative medicine and tissue engineering applications.","subitem_description_type":"Abstract"}]},"item_5_identifier_34":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2241/114071"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"21802723","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.biomaterials.2011.06.034","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 Elsevier Ltd.\n“NOTICE: this is the author's version of a work that was accepted for publication in Biomaterials. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, Vol.32(30),7479-7490, 2011.  DOI:10.1016/j.biomaterials.2011.06.034”"}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0142-9612","subitem_source_identifier_type":"ISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00110092","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sadr, Nasser"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Zhu, Mojun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Osaki, Tatsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kakegawa, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yang, Yunzhi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Moretti, Matteo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda, Junji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Khademhosseini, Ali"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2013-12-25"}],"displaytype":"detail","filename":"Biomaterials_32-30.pdf","filesize":[{"value":"4.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Biomaterials_32-30.pdf","url":"https://tsukuba.repo.nii.ac.jp/record/25204/files/Biomaterials_32-30.pdf"},"version_id":"2989c690-d0fe-47c2-a7da-295245562c78"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures"}]},"item_type_id":"5","owner":"1","path":["808","569"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-10-11"},"publish_date":"2011-10-11","publish_status":"0","recid":"25204","relation_version_is_last":true,"title":["SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-04-27T08:52:57.157113+00:00"}