@article{oai:tsukuba.repo.nii.ac.jp:00025204, author = {福田, 淳二 and Sadr, Nasser and Zhu, Mojun and Osaki, Tatsuya and Kakegawa, Takahiro and Yang, Yunzhi and Moretti, Matteo and Fukuda, Junji and Khademhosseini, Ali}, issue = {30}, journal = {Biomaterials}, month = {Oct}, note = {A major challenge in tissue engineering is to reproduce the native 3D microvascular architecture fundamental for in vivo functions. Current approaches still lack a network of perfusable vessels with native 3D structural organization. Here we present a new method combining self-assembled monolayer (SAM)-based cell transfer and gelatin methacrylate hydrogel photopatterning techniques for microengineering vascular structures. Human umbilical vein cell (HUVEC) transfer from oligopeptide SAM-coated surfaces to the hydrogel revealed two SAM desorption mechanisms: photoinduced and electrochemically triggered. The former, occurs concomitantly to hydrogel photocrosslinking, and resulted in efficient (>97%) monolayer transfer. The latter, prompted by additional potential application, preserved cell morphology and maintained high transfer efficiency of VE-cadherin positive monolayers over longer culture periods. This approach was also applied to transfer HUVECs to 3D geometrically defined vascular-like structures in hydrogels, which were then maintained in perfusion culture for 15 days. As a step toward more complex constructs, a cell-laden hydrogel layer was photopatterned around the endothelialized channel to mimic the vascular smooth muscle structure of distal arterioles. This study shows that the coupling of the SAM-based cell transfer and hydrogel photocrosslinking could potentially open up new avenues in engineering more complex, vascularized tissue constructs for regenerative medicine and tissue engineering applications.}, pages = {7479--7490}, title = {SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures}, volume = {32}, year = {2011} }