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Functions of a hemolysin-like protein in the cyanobacterium Synechocystis sp. PCC 6803
http://hdl.handle.net/2241/113741
http://hdl.handle.net/2241/11374115546149-fe54-4a69-aca9-01eafb5ce420
名前 / ファイル | ライセンス | アクション |
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AM_193-8.pdf (1.2 MB)
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Item type | Journal Article(1) | |||||
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公開日 | 2011-08-22 | |||||
タイトル | ||||||
タイトル | Functions of a hemolysin-like protein in the cyanobacterium Synechocystis sp. PCC 6803 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Sakiyama, Tetsushi
× Sakiyama, Tetsushi× Araie, Hiroya× Suzuki, Iwane× Shiraiwa, Yoshihiro |
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著者別名 |
新家, 弘也
× 新家, 弘也× 鈴木, 石根× 白岩, 善博 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A glucose-tolerant strain of the cyanobacterium Synechocystis sp. PCC 6803, generally referred to as wild type, produces a hemolysin-like protein (HLP) located on the cell surface. To analyze the function of HLP, we constructed a mutant in which the hlp gene was disrupted. The growth rate of the mutant was reduced when the cells were stressed by treatment with CuSO4, CdCl2, ZnCl2, ampicillin, kanamycin, or sorbitol in liquid medium, suggesting that HLP may increase cellular resistance to the inhibitory effects of these compounds. Uptake assays with 109Cd2+ using the silicone–oil layer centrifugation technique revealed that both wild type and mutant cells were labeled with 109Cd2+ within 1 min. Although the total radioactivity was much higher in the wild-type cells, 109Cd2+ incorporation was clearly much higher in the mutant cells after adsorbed 109Cd2+ was removed from the cell surface by washing with EDTA. These findings suggest that HLP functions as a barrier against the adsorption of toxic compounds. | |||||
書誌情報 |
Archives of microbiology 巻 193, 号 8, p. 565-571, 発行日 2011-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0302-8933 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00548209 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 21475984 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s00203-011-0700-2 | |||||
権利 | ||||||
権利情報 | © Springer-Verlag 2011 The original publication is available at www.springerlink.com |
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著者版フラグ | ||||||
値 | author | |||||
出版者 | ||||||
出版者 | Springer-Verlag | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2241/113741 | |||||
識別子タイプ | HDL |