{"created":"2021-11-16T06:32:22.510300+00:00","id":2001877,"links":{},"metadata":{"_buckets":{"deposit":"ccdeb25f-bdf6-4cd6-b14b-cee6a5735510"},"_deposit":{"created_by":14,"id":"2001877","owner":"14","owners":[14],"owners_ext":{"displayname":"大久保光枝","username":"mokubo"},"pid":{"revision_id":0,"type":"depid","value":"2001877"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:02001877","sets":["160:516","3:62:5592:1637043103465"]},"author_link":["132"],"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-6","bibliographicPageEnd":"42","bibliographicPageStart":"35","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"Journal of Molecular Microbiology and Biotechnology","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A cellular slime mold, Dictyostelium discoideum, is an amoeboid organism that has a unique life cycle consisting of distinctly separated vegetative and developmental phases. Thus, this organism presents a rare opportunity in which to examine the effects of bioactive substances on separate cellular activities. In this research, we investigated the effect of a culture extract, termed EMXG, produced by a synthetic microbial consortium. EMXG promoted proliferative response of amoeba cells. It further accelerated the developmental phase, leading to the preferred fruiting body formation from fewer cells. Furthermore, EMXG modulated biological rhythm of this organism, that is, interval of oscillation of cAMP level observed in suspension starvation was significantly shortened. Concomitantly, the level of ERKB, a MAP kinase, was found to oscillate in a similar fashion to that of cAMP. Additionally, ErkB-deficient mutant amoeboid cells did not respond to proliferative stimulation by EMXG. These lines of evidence point to a likelihood that MAP kinase cascade is involved and further that ErkB could be the molecular target of EMXG.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Karger"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"31805558","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1159/000504442","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019 S. Karger AG, Basel.　This is the accepted manuscript version of an article published by S. Karger AG in J Mol Microbiol Biotechnol 2019;29:35–42 including DOI and available on https://doi.org/10.1159/000504442.","subitem_rights_language":"en"}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1464-1801","subitem_source_identifier_type":"PISSN"}]},"item_5_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11563270","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"桑山, 秀一","creatorNameLang":"ja"},{"creatorName":"クワヤマ, ヒデカズ","creatorNameLang":"ja-Kana"},{"creatorName":"KUWAYAMA, hidekazu","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Higashinakagawa, Toru","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-01"}],"displaytype":"detail","filename":"JMMB_29-35.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","url":{"objectType":"fulltext","url":"https://tsukuba.repo.nii.ac.jp/record/2001877/files/JMMB_29-35.pdf"},"version_id":"572c6fb9-d008-4997-9b86-dffee6df4b16"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Life Cycle of <b><i>Dictyostelium discoideum</i></b> Is Accelerated via MAP Kinase Cascade by a Culture Extract Produced by a Synthetic Microbial Consortium","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Life Cycle of <b><i>Dictyostelium discoideum</i></b> Is Accelerated via MAP Kinase Cascade by a Culture Extract Produced by a Synthetic Microbial Consortium","subitem_title_language":"en"}]},"item_type_id":"5","owner":"14","path":["516","1637043103465"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-11-16"},"publish_date":"2021-11-16","publish_status":"0","recid":"2001877","relation_version_is_last":true,"title":["The Life Cycle of <b><i>Dictyostelium discoideum</i></b> Is Accelerated via MAP Kinase Cascade by a Culture Extract Produced by a Synthetic Microbial Consortium"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-07-07T07:55:26.323848+00:00"}