{"created":"2021-10-29T02:43:45.988033+00:00","id":2001717,"links":{},"metadata":{"_buckets":{"deposit":"3013efc4-51c6-48ce-ae5b-5004a0df342e"},"_deposit":{"created_by":14,"id":"2001717","owner":"14","owners":[14],"owners_ext":{"displayname":"大久保光枝","username":"mokubo"},"pid":{"revision_id":0,"type":"depid","value":"2001717"},"status":"published"},"_oai":{"id":"oai:tsukuba.repo.nii.ac.jp:02001717","sets":["2780:335","2780:922","3:62:5595:7193"]},"author_link":["124027","32"],"item_5_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"49","bibliographicPageStart":"44","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Molecular Therapy - Methods & Clinical Development","bibliographic_titleLang":"en"}]}]},"item_5_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To establish an efficient, safe immunosuppressive regimen of adeno-associated vector (AAV)-mediated gene therapy for Duchenne muscular dystrophy (DMD), we evaluated the effect of tacrolimus (FK506) on skeletal muscle transduction with AAV8 and AAV9 vectors expressing the LacZ and microdystrophin (M3) genes labeled by FLAG. We utilized 3- to 4-year-old Macaca fascicularis, screened for neutralizing antibodies against AAV. 3 days before AAV injection and throughout the experiment, 0.06 mg/kg tacrolimus was intravenously administered. A viral suspension of 1 × 1013 viral genomes/muscle was intramuscularly injected bilaterally at the tibialis anterior and biceps brachii muscles, which were biopsied at 8, 16, 24, and 42 weeks after injection. Without tacrolimus, AAV8- and AAV9-mediated LacZ expression disappeared 8 and 16 weeks after transduction, respectively. With tacrolimus, AAV8/9-mediated LacZ expression persisted for at least 42 weeks after injection. At 42 weeks after AAV8CMVLacZ and AAV9CMVLacZ injection, nearly 50% and 17% of muscle fibers were positive for β-galactosidase, respectively. AAV8/9-mediated M3-FLAG expression lasted for up to 42 weeks using tacrolimus. No significant generalized toxicity was observed in any monkey. These results indicate that tacrolimus administration regulated the immune response to transgenes and truncated microdystrophin in normal primates and may enhance the benefits of AAV-mediated gene therapy for DMD.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_5_publisher_27":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cell Press","subitem_publisher_language":"en"}]},"item_5_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"32577431","subitem_relation_type_select":"PMID"}}]},"item_5_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.omtm.2020.05.012","subitem_relation_type_select":"DOI"}}]},"item_5_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).","subitem_rights_language":"en"}]},"item_5_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_5_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"23290501","subitem_source_identifier_type":"EISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石井, 亜紀子","creatorNameLang":"ja"},{"creatorName":"イシイ, アキコ","creatorNameLang":"ja-Kana"},{"creatorName":"ISHII, Akiko","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Okada, Hironori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hayashita-Kinoh, Hiromi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shin, Jin-Hong","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"玉岡, 晃","creatorNameLang":"ja"},{"creatorName":"タマオカ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"TAMAOKA, Akira","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Okada, Takashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takeda, Shin’ichi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2021-10-29"}],"displaytype":"detail","filename":"MTMCD_18-44.pdf","filesize":[{"value":"956 KB"}],"format":"application/pdf","licensetype":"license_5","url":{"objectType":"fulltext","url":"https://tsukuba.repo.nii.ac.jp/record/2001717/files/MTMCD_18-44.pdf"},"version_id":"b67cd6bb-952e-4333-a24d-696f4655d7dd"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"rAAV8 and rAAV9-Mediated Long-Term Muscle Transduction with Tacrolimus (FK506) in Non-Human Primates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"rAAV8 and rAAV9-Mediated Long-Term Muscle Transduction with Tacrolimus (FK506) in Non-Human Primates","subitem_title_language":"en"}]},"item_type_id":"5","owner":"14","path":["922","335","7193"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-10-29"},"publish_date":"2021-10-29","publish_status":"0","recid":"2001717","relation_version_is_last":true,"title":["rAAV8 and rAAV9-Mediated Long-Term Muscle Transduction with Tacrolimus (FK506) in Non-Human Primates"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2022-04-27T10:02:11.370405+00:00"}