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Analysis and effect of conventional flasks in shaking culture of Escherichia coli
http://hdl.handle.net/2241/0002001210
http://hdl.handle.net/2241/000200121098a786d8-20fc-41cd-9c6a-5185106a11ac
名前 / ファイル | ライセンス | アクション |
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AMB_10-1.pdf (1.1 MB)
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Item type | Journal Article(1) | |||||||
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公開日 | 2021-09-06 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Analysis and effect of conventional flasks in shaking culture of Escherichia coli | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||
タイプ | journal article | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 |
Takahashi, Masato
× Takahashi, Masato
× 青柳, 秀紀 |
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | The circulation direct monitoring and sampling system (CDMSS) is used as a monitoring device for CO2 and O2 concentrations of bypass type in shake-culture flask. The CDMSS could measure kLa, an index for evaluating the performance of aerobic culture incubators, and kG, an indicator of the degree of CO2 ventilation in the flask gas phase. We observed that cylindrical flasks provided a different culture environment, yielded a much higher kG than the Erlenmeyer and Sakaguchi flasks, and yielded kLa equivalent to that by Erlenmeyer flask by setting the ring-type baffle appropriately. Baffled cylindrical flask used for Escherichia coli K12 IFO3301 shake culture maintained lower CO2 concentrations in the headspace than conventional flasks; therefore, CO2 accumulation in the culture broth could be suppressed. Cell growth in baffled cylindrical flask (with kLa equivalent to that of the Erlenmeyer flask) was about 1.3 and 1.4 times that in the Erlenmeyer and Sakaguchi flasks, respectively. This study focused on the batch culture at the flask scale and designed the headspace environment with low CO2 accumulation. Therefore, we conclude that redesign of flasks based on kLa and kG may contribute to a wide range of fields employing microorganism culture. | |||||||
言語 | en | |||||||
書誌情報 |
en : AMB Express 巻 10, 号 1, p. 77, 発行日 2020-04-19 |
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ISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 2191-0855 | |||||||
PubMed番号 | ||||||||
識別子タイプ | PMID | |||||||
関連識別子 | 32307613 | |||||||
DOI | ||||||||
識別子タイプ | DOI | |||||||
関連識別子 | https://doi.org/10.1186/s13568-020-01013-7 | |||||||
権利 | ||||||||
言語 | en | |||||||
権利情報 | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/. | |||||||
著者版フラグ | ||||||||
値 | publisher | |||||||
出版者 | ||||||||
言語 | en | |||||||
出版者 | Springer Open |