@article{oai:tsukuba.repo.nii.ac.jp:00018175, author = {Noguchi, Atsushi and Ishiwata, Hiroshi and Itoh, Masahide and 伊藤, 雅英 and Yatagai, Toyohiko}, issue = {16}, journal = {Optics Communications}, month = {Aug}, note = {While developing a three-dimensional (3-D) measurement technique for the retardation-modulated differential interference contrast (RM-DIC) microscope, we encountered a problem. The problem was that the measurement range was restricted in λ because it applies weak phase approximation. To overcome this drawback, we propose a 3-D reconstruction method with z-axis scanning. This technique needs high optical sectioning, like confocal microscopy. We investigated the characteristic of optical sectioning in a DIC microscope, then we confirmed experimentally that a DIC microscope has high optical sectioning. We also confirmed that a RM-DIC microscope has higher optical sectioning. By combining the optical sectioning of a RM-DIC microscope and z-scanning, we developed a new 3-D reconstruction method. This novel technique overcomes the observed problem as the measurement range is increased to micron order.}, pages = {3223--3230}, title = {Optical sectioning in differential interference contrast microscopy}, volume = {282}, year = {2009}, yomi = {イトウ, マサヒデ} }