2024-03-28T17:50:36Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00047717
2022-04-27T09:19:47Z
2780:5925
3:62:5589:6906
SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology
錦井, 秀和
Abdelmoez, Mahmoud N.
Iida, Kei
Oguchi, Yusuke
Nishikii, Hidekazu
Yokokawa, Ryuji
Kotera, Hidetoshi
Uemura, Sotaro
Santiago, Juan G.
Shintaku, Hirofumi
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.
journal article
BioMed Central
2018-06
application/pdf
Genome biology
19
66
1474-760X
AA12050817
https://tsukuba.repo.nii.ac.jp/record/47717/files/GB_19-66.pdf
eng
29871653
10.1186/s13059-018-1446-9
© The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.