2024-03-29T02:27:14Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00045932
2022-04-27T09:17:40Z
160:6459
3:62:5592:6458
Heterologous and High Production of Ergothioneine in Escherichia coli
大津, 厳生
Osawa, Ryo
Kamide, Tomoyuki
Satoh, Yasuharu
Kawano, Yusuke
Ohtsu, Iwao
Dairi, Tohru
Ergothioneine (ERG) is a histidine-derived thiol compound suggested to function as an antioxidant and cytoprotectant in humans. Therefore, experimental trials have been conducted applying ERG from mushrooms in dietary supplements and as a cosmetic additive. However, this method of producing ERG is expensive; therefore, alternative methods for ERG supply are required. Five Mycobacterium smegmatis genes, egtABCDE, have been confirmed to be responsible for ERG biosynthesis. This enabled us to develop practical fermentative ERG production by microorganisms. In this study, we carried out heterologous and high-level production of ERG in Escherichia coli using the egt genes from M. smegmatis. By high production of each of the Egt enzymes and elimination of bottlenecks in the substrate supply, we succeeded in constructing a production system that yielded 24 mg/L (104 μM) secreted ERG.
journal article
American Chemical Society
2018-02
application/pdf
Journal of agricultural and food chemistry
5
66
1191
1196
0021-8561
AA00250794
https://tsukuba.repo.nii.ac.jp/record/45932/files/JAFC_66-5.pdf
eng
29276826
10.1021/acs.jafc.7b04924
© 2017 American Chemical Society
This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.