2024-03-29T10:06:22Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00043463
2022-04-27T09:14:33Z
160:4856
3:62:5297:4936
Phenylpropanoid 2,3-dioxygenase involved in the cleavage of the ferulic acid side chain to form vanillin and glyoxylic acid in Vanilla planifolia
根岸, 紀
Negishi, Osamu
Negishi, Yukiko
Enzyme catalyzing the cleavage of the phenylpropanoid side chain was partially purified by ion exchange and gel filtration column chromatography after (NH4)2SO4 precipitation. Enzyme activities were dependent on the concentration of dithiothreitol (DTT) or glutathione (GSH) and activated by addition of 0.5 mM Fe2+. Enzyme activity for ferulic acid was as high as for 4-coumaric acid in the presence of GSH, suggesting that GSH acts as an endogenous reductant in vanillin biosynthesis. Analyses of the enzymatic reaction products with quantitative NMR (qNMR) indicated that an amount of glyoxylic acid (GA) proportional to vanillin was released from ferulic acid by the enzymatic reaction. These results suggest that phenylpropanoid 2,3-dioxygenase is involved in the cleavage of the ferulic acid side chain to form vanillin and GA in Vanilla planifolia.
journal article
Taylor & Francis
2017-07
application/pdf
Bioscience, biotechnology, and biochemistry
9
81
1732
1740
0916-8451
AA10824164
https://tsukuba.repo.nii.ac.jp/record/43463/files/BBB_81-9.pdf
eng
28743207
10.1080/09168451.2017.1353402
This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, biotechnology, and biochemistry on 26 Jul 2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1353402.