2024-03-29T07:11:55Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00041668
2022-04-27T09:12:36Z
160:1470
3:62:5595:642
Stimulation of microtubule-based transport by nucleation of microtubules on pigment granules
臼井, 健郎
Semenova, Irina
Gupta, Dipika
Usui, Takeo
Hayakawa, Ichiro
Cowan, Ann
Rodionov, Vladimir
Microtubule (MT)-based transport can be regulated through changes in organization of MT transport tracks, but the mechanisms that regulate these changes are poorly understood. In Xenopus melanophores, aggregation of pigment granules in the cell center involves their capture by the tips of MTs growing toward the cell periphery, and granule aggregation signals facilitate capture by increasing the number of growing MT tips. This increase could be explained by stimulation of MT nucleation either on the centrosome or on the aggregate of pigment granules that gradually forms in the cell center. We blocked movement of pigment granules to the cell center and compared the MT-nucleation activity of the centrosome in the same cells in two signaling states. We found that granule aggregation signals did not stimulate MT nucleation on the centrosome but did increase MT nucleation activity of pigment granules. Elevation of MT-nucleation activity correlated with the recruitment to pigment granules of a major component of MT-nucleation templates, γ-tubulin, and was suppressed by γ-tubulin inhibitors. We conclude that generation of new MT transport tracks by concentration of the leading pigment granules provides a positive feedback loop that enhances delivery of trailing granules to the cell center.
journal article
American Society for Cell Biology
2017-06
application/pdf
Molecular biology of the cell
11
28
1418
1425
1059-1524
AA10830484
https://tsukuba.repo.nii.ac.jp/record/41668/files/MBC_28-11-1418.pdf
eng
28381426
10.1091/mbc.E16-08-0571
© 2017 Semenova et al.
This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.