2024-03-29T05:54:32Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00038245
2022-08-04T06:42:16Z
2780:1495
2780:1501
2780:1639975837591
2780:171
2780:2762
2780:5141
3:62:5592:5140
MicroRNA-155-5p is associated with oral squamous cell carcinoma metastasis and poor prognosis
Baba, Osamu
Hasegawa, Shogo
Nagai, Hiroki
内田, 文彦
ウチダ, フミヒコ
UCHIDA, Fumihiko
Yamatoji, Masanobu
菅野, 直美
カンノ, ナオミ
KANNO, Naomi
山縣, 憲司
ヤマガタ, ケンジ
YAMAGATA, Kenji
酒井, 俊
サカイ, サトシ
SAKAI, Satoshi
柳川, 徹
ヤナガワ, トオル
YANAGAWA, Toru
武川, 寛樹
ブカワ, ヒロキ
BUKAWA, Hiroki
Background<br/>Abnormal miRNA expression was recently implicated in the metastasis of oral squamous cell carcinoma (OSCC) and with a poor prognosis. The initiation of the invasion-metastasis cascade involves epithelial-mesenchymal transition (EMT). Our aim was to clarify how miRNA, especially miR-155-5p misexpression contributes to OSCC metastasis through EMT.<br/><br/>Methods<br/>We collected tumor samples from 73 subjects with OSCC. The samples were analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR), and correlations between miR-155-5p levels and clinical characteristics were investigated. OSCC cell lines were analyzed by miRNA microarray and by transfection with a miR-155-5p mimic or inhibitor, followed by proliferation and wound-healing migration assays. qRT-PCR analyses of EMT makers in cells transfected with miR-155-5p inhibitor were performed.<br/><br/>Results<br/>We found high miR-155-5p expression in tissue samples from subjects with OSCC that had metastasized to cervical lymph nodes. HSC-3 cells also strongly expressed miR-155-5p. The epithelial marker E-cadherin was strongly expressed in HSC-3 cells transfected with miR-155-5p inhibitor, and we observed elevated SOCS1 and decreased STAT3 expression in these cells.<br/><br/>Conclusions<br/>Our results suggest that miR-155-5p causes OSCC to metastasize, and could serve as a novel therapeutic target for OSCC.
journal article
John Wiley & Sons Ltd
Journal of Oral Pathology & Medicine is the official publication of the International Association of Oral Pathologists, British Society for Oral Medicine, British Society of Oral and Maxillofacial Pathology, Scandinavian Fellowship of Oral Pathology and Oral Medicine, and Japanese Society of Oral Pathology.
2016-04
application/pdf
Journal of oral pathology & medicine
4
45
248
255
0904-2512
AA10715555
https://tsukuba.repo.nii.ac.jp/record/38245/files/JOPM_45-4.pdf
eng
26307116
https://doi.org/10.1111/jop.12351
© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
This is the peer reviewed version of the following article: J Oral Pathol Med (2016) 45: 248-255, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/jop.12351/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
open access