2024-03-28T13:18:03Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00029065
2022-04-27T08:57:24Z
160:852
3:62:5298:1323
(−)-Epigallocatechin gallate suppresses adipocyte differentiation through the MEK/ERK and PI3K/Akt pathways
坂本, 和一
Kim, Hyojung
Sakamoto, Kazuichi
EGCG [(−)-epigallocatechin gallate], tea catechin, is one of the compounds that has been reported to act against obesity and diabetes. To determine the effect of EGCG on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different catechins. Oil Red O staining showed significantly reduced intracellular lipid accumulation, especially with EGCG. Cell cycle analysis showed that EGCG inhibited cell proliferation by disturbing the cell cycle during the clonal expansion of 3T3-L1. RT-PCR (real-time PCR) demonstrated that EGCG noticeably reduced mRNA expression of PPARγ (peroxisome proliferator-activated receptor γ), C/EBPα (CCAAT/enhancer-binding protein α) and FoxO1 (forkhead box class O1). EGCG also caused a significant decrease in the transcription of FoxO1 – the forkhead transcription factor class O1 involved in adipocyte differentiation – via the PI3K (phosphoinositide 3-kinase)/Akt and MEK [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase] pathways. These results suggest that EGCG suppresses the clonal expansion of adipocytes by inactivating FoxO1 via insulin signalling and stress-dependent MAPK pathways.
journal article
Portland Press Limited
2012-02
application/pdf
Cell biology international
2
36
147
153
http://hdl.handle.net/2241/119305
1065-6995
AA10882014
https://tsukuba.repo.nii.ac.jp/record/29065/files/CBI_36-2.pdf
eng
21902673
10.1042/CBI20110047
© The Author(s) Journal compilation © 2012 Portland Press Limited The Version of Record (VoR) is available at http://www.cellbiolint.org