2024-03-29T15:54:06Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00027761
2022-04-27T08:54:46Z
160:1474
160:523
3:62:5298:387
Down regulation effect of Rosmarinus officinalis polyphenols on cellular stress proteins in rat pheochromocytoma PC12 cells
韓, 畯奎
礒田, 博子
Omri, Abdelfatteh E. L.
Han, Junkyu
Abdrabbah, Manef Ben
Isoda, Hiroko
Polyphenols are known to exhibit wide spectrum of benefit for brain health and to protect from several neurodegenerative diseases. The present study was sought to determine the neuroprotective effects of Rosmarinus officinalis’ polyphenols (luteolin, carnosic acid, and rosmarinic acid) through the investigation of stress-related proteins. We carried out measurement of the expression of heat-shock protein (Hsp) 47 promoter in heat stressed Chinese hamster ovary transfected cells. We performed proteomic analysis and confirmed gene expression by real time PCR in PC12 cells. Results showed that these compounds modulated significant and different effects on the expression of 4 stress-related proteins: heat shock protein 90 α (Hsp90), Transitional endoplasmic reticulum ATPase (VCP/p97), Nucleoside diphosphate kinase (NDK), and Hypoxia up-regulated protein 1 (HYOU1)) at translational and post translational levels in PC12 cells and they downregulated the expression of Hsp47 activity in Chinese hamster transformed cells. These findings suggest that luteolin, carnosic acid, and rosmarinic acid may modulate the neuroprotective defense system against cellular stress insults and increase neuro-thermotolerance.
journal article
Springer Science+Business Media B.V.
2012-05
application/pdf
Cytotechnology
3
64
231
240
http://hdl.handle.net/2241/117705
0920-9069
AA10678299
https://tsukuba.repo.nii.ac.jp/record/27761/files/Cy_64-3.pdf
eng
21861121
10.1007/s10616-011-9352-y
© Springer Science+Business Media B.V. 2011
The original publication is available at www.springerlink.com