2024-03-28T15:55:12Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00026391
2022-04-27T08:54:10Z
117:1941
117:811
3:62:5601:1133
Novel redox nanomedicine improves gene expression of polyion complex vector
池田, 豊
長崎, 幸夫
Toh, Kazuko
Yoshitomi, Toru
Ikeda, Yutaka
Nagasaki, Yukio
Gene therapy has generated worldwide attention as a new medical technology. While non-viral gene vectors are promising candidates as gene carriers, they have several issues such as toxicity and low transfection efficiency. We have hypothesized that the generation of reactive oxygen species (ROS) affects gene expression in polyplex supported gene delivery systems. The effect of ROS on the gene expression of polyplex was evaluated using a nitroxide radical-containing nanoparticle (RNP) as an ROS scavenger. When polyethyleneimine (PEI)/pGL3 or PEI alone was added to the HeLa cells, ROS levels increased significantly. In contrast, when (PEI)/pGL3 or PEI was added with RNP, the ROS levels were suppressed. The luciferase expression was increased by the treatment with RNP in a dose-dependent manner and the cellular uptake of pDNA was also increased. Inflammatory cytokines play an important role in ROS generation in vivo. In particular, tumor necrosis factor (TNF)-α caused intracellular ROS generation in HeLa cells and decreased gene expression. RNP treatment suppressed ROS production even in the presence of TNF-α and increased gene expression. This anti-inflammatory property of RNP suggests that it may be used as an effective adjuvant for non-viral gene delivery systems.
journal article
IOP PUBLISHING
2011
application/pdf
Science and technology of advanced materials
6
12
065001
http://hdl.handle.net/2241/115376
1468-6996
AA11539797
https://tsukuba.repo.nii.ac.jp/record/26391/files/STAM_12-6.pdf
eng
10.1088/1468-6996/12/6/065001
© 2011 National Institute for Materials Science