2024-03-28T20:40:53Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00025841
2022-04-27T08:53:31Z
160:528
3:62:5602:1664
Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter
江面, 浩
Hirai, Tadayoshi
Kim, You-Wang
Kato, Kazuhisa
Hiwasa-Tanase, Kyoko
Ezura, Hiroshi
The E8 promoter, a tomato fruit-ripeningspecific
promoter, and the CaMV 35S promoter, a
constitutive promoter, were used to express the
miraculin gene encoding the taste-modifying protein
in tomato. The accumulation of miraculin protein and
mRNA was compared among transgenic tomatoes
expressing the miraculin gene driven by these
promoters. Recombinant miraculin protein predominantly
accumulated in transgenic tomato lines using
the E8 promoter (E8-MIR) only at the red fruit stage.
The accumulations were almost uniform among all
fruit tissues. When the 35S promoter (35S-MIR) was
used, miraculin accumulation in the exocarp was
much higher than in other tissues, indicating that the
miraculin accumulation pattern can be regulated by
using different types of promoters. We also discuss
the potential of the E8-MIR lines for practical use.
journal article
Springer
2011-12
application/pdf
Transgenic research
6
20
1285
1292
http://hdl.handle.net/2241/114758
0962-8819
AA10873487
https://tsukuba.repo.nii.ac.jp/record/25841/files/TR_20-6.pdf
eng
21359850
10.1007/s11248-011-9495-9
© Springer Science+Business Media B.V. 2011.
The final publication is available at www.springerlink.com