2024-03-28T12:30:12Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00039514
2022-04-27T09:09:40Z
160:855
160:857
3:62:5297:376
Construction of a cyanobacterium synthesizing cyclopropane fatty acids
白岩, 善博
鈴木, 石根
Machida, Shuntaro
Shiraiwa, Yoshihiro
Suzuki, Iwane
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Microalgae have received much attention as a next-generation source of biomass energy. However, most of the fatty acids (FAs) from microalgae are multiply unsaturated; thus, the biofuels derived from them are fluid, but vulnerable to oxidation. In this study, we attempted to synthesize cyclopropane FAs in the cyanobacterium Synechocystis sp. PCC 6803 by expressing the cfa gene for cyclopropane FA synthase from Escherichia coli with the aim of producing FAs that are fluid and stable in response to oxidization. We successfully synthesized cyclopropane FAs in Synechocystis with a yield of ~ 30% of total FAs. Growth of the transformants was altered, particularly at low temperatures, but photosynthesis and respiration were not significantly affected. C16:1∆ 9 synthesis in the desA−/desD− strain by expression of the desC2 gene for sn-2 specific ∆ 9 desaturase positively affected growth at low temperatures via promotion of various cellular processes, with the exceptions of photosynthesis and respiration. Estimation of the apparent activities of desaturases suggested that some acyl-lipid desaturases might recognize the lipid side chain.
Elsevier
2016-09
eng
journal article
http://hdl.handle.net/2241/00144182
https://tsukuba.repo.nii.ac.jp/records/39514
27263419
10.1016/j.bbalip.2016.05.012
1388-1981
AA11300409
Biochimica et biophysica acta. Molecular and cell biology of lipids
1861
9
980
987
https://tsukuba.repo.nii.ac.jp/record/39514/files/BBA-MCBL_1861-9.pdf
application/pdf
1.2 MB
2017-10-01