2024-03-29T14:12:47Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00039165
2023-02-09T02:15:19Z
160:5362
3:62:5298:5294
Nociceptor-Enriched Genes Required for Normal Thermal Nociception
本庄, 賢
ホンジョウ, ケン
HONJO, Ken
Mauthner, Stephanie?E.
Wang, Yu
Skene, J.H.?Pate
Tracey, W.?Daniel
open access
(c) 2016 The Author(s).
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Here, we describe a targeted reverse genetic screen for thermal nociception genes in Drosophila larvae. Using laser capture microdissection and microarray analyses of nociceptive and non-nociceptive neurons, we identified 275 nociceptor-enriched genes. We then tested the function of the enriched genes with nociceptor-specific RNAi and thermal nociception assays. Tissue-specific RNAi targeted against 14 genes caused insensitive thermal nociception while targeting of 22 genes caused hypersensitive thermal nociception. Previously uncategorized genes were named for heat resistance (i.e., boilerman, fire dancer, oven mitt, trivet, thawb, and bunker gear) or heat sensitivity (firelighter, black match, eucalyptus, primacord, jet fuel, detonator, gasoline, smoke alarm, and jetboil). Insensitive nociception phenotypes were often associated with severely reduced branching of nociceptor neurites and hyperbranched dendrites were seen in two of the hypersensitive cases. Many genes that we identified are conserved in mammals.
Cell Press
2016-07
eng
journal article
VoR
http://hdl.handle.net/2241/00143832
https://tsukuba.repo.nii.ac.jp/records/39165
27346357
https://doi.org/10.1016/j.celrep.2016.06.003
2211-1247
Cell Reports
16
2
295
303
https://tsukuba.repo.nii.ac.jp/record/39165/files/CR_16-2.pdf
application/pdf
2.6 MB
2016-09-09