2024-03-29T01:35:57Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00038303
2023-07-12T06:23:03Z
2770:2579
3:62:5297:5198
The neostriatum: two entities, one structure?
ミハエル, ラザルス
Lopez-Huerta, Violeta G.
Nakano, Yoko
Bausenwein, Johannes
Jaidar, Omar
Cherassse, Yoan
Garcia-Munoz, Marianela
Arbuthnott, Gordon
Lazarus , Michael
© The Author(s) 2015. This article is published with open access at Springerlink.com
The striosome (or patch) was first identified with anatomical techniques as neurons organized in a three-dimensional labyrinth inserted in and interdigitating the rest of neostriatum: the matrix. Striosome and matrix rapidly became known as two neuronal compartments expressing different biochemical markers, embryonic development and afferent and efferent connectivity. In spite of extensive intrinsic neuronal axonal and dendritic extensions supposed to exchange information between matrix and striosomes, evidence suggested the presence of independent areas. Here, we report that indeed these two areas do not exchange synaptic information. We used genetic expression of channel rhodopsin 2 carried by adeno-associated virus serotype 10 (AAVrh10) that only expresses in neurons of the matrix compartment. Whole-cell patchclamp recordings of matrix neurons activated by light pulses consistently produced inhibitory postsynaptic currents (IPSCs), but the same manipulation did not evoke IPSCs in striosome neurons. The matrix contains both direct and indirect striatal output pathways. By targeting striatal matrix expression of designer receptors exclusively activated by a designer drug (DREADD) hM3di carried by AAVrh10, we were able to inhibit the matrix neuronal compartment of the dorsolateral striatum during performance of a learned single-pellet reach-to-grasp task. As expected, inhibition of matrix neurons by systemic administration of DREADD agonist clozapine-n-oxide interfered with performance of the learned task.
An erratum to this article can be found at http://dx.doi.org/10.1007/s00429-015-1017-8
Springer Heidelberg
2016-04
eng
journal article
http://hdl.handle.net/2241/00142315
https://tsukuba.repo.nii.ac.jp/records/38303
25652680
10.1007/s00429-015-1000-4
1863-2653
Brain structure and function
221
3
1737
1749
https://tsukuba.repo.nii.ac.jp/record/38303/files/BRAIN S&F 221-3.pdf
application/pdf
5.2 MB
2016-06-17
https://tsukuba.repo.nii.ac.jp/record/38303/files/BRAIN S&F 221-3_erratum.pdf
application/pdf
192.7 kB
2018-12-17