2024-03-28T22:22:29Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00036404
2022-04-27T09:05:28Z
2780:1269
2780:1270
2780:4839
3:62:5586:4838
Contribution of the Runx1 transcription factor to axonal pathfinding and muscle innervation by hypoglossal motoneurons
尾崎, 繁
増田, 知之
志賀, 隆
Yoshikawa, Masaaki
Hirabayashi, Mizuki
Ito, Ryota
Ozaki, Shigeru
Aizawa, Shin
Masuda, Tomoyuki
Senzaki, Kouji
Shiga, Takashi
© 2015 Wiley Periodicals, Inc.
This is the peer reviewed version of the following article: Develop Neurobiol 75: 1295–1314, 2015, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/dneu.22285. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
The runt-related transcription factor Runx1 contributes to cell type specification and axonal targeting projections of the nociceptive dorsal root ganglion neurons. Runx1 is also expressed in the central nervous system, but little is known of its functions in brain development. At mouse embryonic day (E) 17.5, Runx1-positive neurons were detected in the ventrocaudal subdivision of the hypoglossal nucleus. Runx1-positive neurons lacked calcitonin gene-related peptide (CGRP) expression, whereas Runx1-negative neurons expressed CGRP. Expression of CGRP was not changed in Runx1-deficient mice at E17.5, suggesting that Runx1 alone does not suppress CGRP expression. Hypoglossal axon projections to the intrinsic vertical (V) and transverse (T) tongue muscles were sparser in Runx1-deficient mice at E17.5 compared to age-matched wild-type littermates. Concomitantly, vesicular acetylcholine transporter-positive axon terminals and acetylcholine receptor clusters were less dense in the V and T tongue muscles of Runx1-deficient mice. These abnormalities in axonal projection were not caused by a reduction in the total number hypoglossal neurons, failed synaptogenesis, or tongue muscles deficits. Our results implicate Runx1 in the targeting of ventrocaudal hypoglossal axons to specific tongue muscles. However, Runx1 deficiency did not alter neuronal survival or the expression of multiple motoneuron markers as in other neuronal populations. Thus, Runx1 appears to have distinct developmental functions in different brain regions.
Wiley
2015-11
eng
journal article
http://hdl.handle.net/2241/00131945
https://tsukuba.repo.nii.ac.jp/records/36404
25762373
10.1002/dneu.22285
1932-8451
AA12195729
Developmental neurobiology
75
11
1295
1314
https://tsukuba.repo.nii.ac.jp/record/36404/files/DN_75-11.pdf
application/pdf
7.5 MB
2016-12-01