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https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00035816
2022-04-27T09:04:48Z
117:1934
2780:954
2780:960
3:62:5592:4743
Multimodal and multiplex spectral imaging of rat cornea ex vivo using a white-light laser source
加治, 優一
大鹿, 哲郎
加納, 英明
Segawa, Hiroki
Kaji, Yuichi
Leproux, Philippe
Couderc, Vincent
Ozawa, Takeaki
Oshika, Tetsuro
Kano, Hideaki
© 2014 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
This is the accepted version of the following article: J. Biophoton., 8: 705–713, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbio.201400059/full.
Multimodal nonlinear spectral images of rat cornea at corneal epithelium and corneal stroma in the in-plane (XY) direction. With use of the combinational analysis of different nonlinear optical processes, detailed molecular structural information is available without staining or labelling.
We applied our multimodal nonlinear spectral imaging microscope to the measurement of rat cornea. We successfully obtained multiple nonlinear signals of coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and second harmonic generation (SHG). Depending on the nonlinear optical processes, the cornea tissue was visualized with different image contrast mechanism simultaneously. Due to white-light laser excitation, multiplex CARS and TSFG spectra were obtained. Combined multimodal and spectral analysis clearly elucidated the layered structure of rat cornea with molecular structural information. This study indicates that our multimodal nonlinear spectral microscope is a promising bioimaging method for tissue study.
Wiley
2015-09
eng
journal article
http://hdl.handle.net/2241/00129055
https://tsukuba.repo.nii.ac.jp/records/35816
25378211
10.1002/jbio.201400059
1864-0648
AA12634206
Journal of Biophotonics
8
9
705
713
https://tsukuba.repo.nii.ac.jp/record/35816/files/JOB_8-9.pdf
application/pdf
471.7 kB
2016-10-01