2024-03-28T15:01:34Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00029222
2022-04-27T08:57:36Z
2780:983
29:342
3:62:5592:2044
B23/nucleophosmin is involved in regulation of adenovirus chromatin structure at late infection stages, but not in virus replication and transcription
小松, 哲郎
奧脇, 暢
永田, 恭介
Samad, Mohammad Abdus
Komatsu, Tetsuro
Okuwaki, Mitsuru
Nagata, Kyosuke
© 2012 SGM
B23/nucleophosmin has been identified in vitro as a stimulatory factor for replication of adenovirus DNA complexed with viral basic core proteins. In the present study, the in vivo function of B23 in the adenovirus life cycle was studied. It was found that both the expression of a decoy mutant derived from adenovirus core protein V that tightly associates with B23 and small interfering RNA-mediated depletion of B23 impeded the production of progeny virions. However, B23 depletion did not significantly affect the replication and transcription of the virus genome. Chromatin immunoprecipitation analyses revealed that B23 depletion significantly increased the association of viral DNA with viral core proteins and cellular histones. These results suggest that B23 is involved in the regulation of association and/or dissociation of core proteins and cellular histones with the virus genome. In addition, these results suggest that proper viral chromatin assembly, regulated in part by B23, is crucial for the maturation of infectious virus particles.
Society for General Microbiology
2012-06
eng
journal article
http://hdl.handle.net/2241/119515
https://tsukuba.repo.nii.ac.jp/records/29222
22337638
10.1099/vir.0.036665-0
0022-1317
AA00698722
Journal of general virology
93
6
1328
1338
https://tsukuba.repo.nii.ac.jp/record/29222/files/JGV_93-6.pdf
application/pdf
4.2 MB
2013-12-25