2024-03-29T00:46:44Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00027085
2022-04-27T08:54:52Z
2780:1976
2780:984
2780:987
3:62:5595:431
7131:543
7131:988
Isolation and characterization of naïve follicular dendritic cells
本多, 伸一郎
小田, ちぐさ
田原, 聡子
渋谷, 和子
渋谷, 彰
Usui, Kenta
Honda, Shin-ichiro
Yoshizawa, Yuichi
Nakahashi-Oda, Chigusa
Tahara-Hanaoka, Satoko
Shibuya, Kazuko
Shibuya, Akira
© 2011 Elsevier Ltd.
NOTICE: this is the author’s version of a work that was accepted for publication in Molecular immunology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, 50(3),(2012) DOI:10.1016/j.molimm.2011.11.010
Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naïve mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for ∼0.2% of the spleen cells of naïve mice, were CD45−, FDC-M2+, and ICAM-1+, and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation.
Elsevier
2012-03
eng
journal article
http://hdl.handle.net/2241/116885
https://tsukuba.repo.nii.ac.jp/records/27085
22189408
10.1016/j.molimm.2011.11.010
0161-5890
AA00307483
Molecular immunology
50
3
172
176
https://tsukuba.repo.nii.ac.jp/record/27085/files/MI_50-3.pdf
application/pdf
831.8 kB
2013-12-25