2024-03-28T18:25:35Z
https://tsukuba.repo.nii.ac.jp/oai
oai:tsukuba.repo.nii.ac.jp:00025202
2022-04-27T08:52:57Z
160:1260
160:1474
160:523
3:62:5598:1646
Inhibitory Effect of Acteoside Isolated from Cistanche tubulosa on Chemical Mediator Release and Inflammatory Cytokine Production by RBL-2H3 and KU812 Cells
山田, パリーダ
韓, 畯奎
繁森, 英幸
礒田, 博子
Yamada, Parida
Iijima, Rieko
Han, Junkyu
Shigemori, Hideyuki
Yokota, Shinichi
Isoda, Hiroko
© Georg Thieme Verlag
The immediate-type allergic reaction is involved in many allergic diseases such as asthma, allergic rhinitis, and sinusitis. In this study, we investigated the effect of acteoside extracted from Cistanche tubulosa (Schrenk) R. Wight on the basophilic cell-mediated allergic reaction. The effect of acteoside on β-hexosaminidase release and intracellular [Ca2+]i level from rat basophilic leukemia (RBL-2H3) cells was determined. Also, ELISA was used to determine the level of histamine, tumor necrosis factor (TNF)-α, and interleukin (IL)-4 on human basophilic (KU812) cells. The effect of acteoside on basophilic cell viability was determined using the 3-[4,5-dimethylthiazolyl]-2,5-diphenyltetrazolium bromide (MTT) assay. These results indicated that 0.1-10.0 µg/mL acteoside inhibits the release of β-hexosaminidase and [Ca2+]i influx from IgE-mediated RBL-2H3 cells. Moreover, acteoside inhibited histamine release, TNF-α, and IL-4 production in a dose-dependent manner from calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA) or compound 48/80-stimulated KU812 cells. Our findings provide evidence that acteoside inhibits basophilic cell-derived immediate-type and delayed-type allergic reactions. This is the first report describing antiallergic activity of acteoside extracted from Cistanche tubulosa on basophilic cells.
Georg Thieme Verlag
2010-10
eng
journal article
http://hdl.handle.net/2241/114068
https://tsukuba.repo.nii.ac.jp/records/25202
20354949
10.1055/s-0030-1249775
0032-0943
AA00348196
Planta medica
76
14
1512
1518
https://tsukuba.repo.nii.ac.jp/record/25202/files/PM_74-14.pdf
application/pdf
384.1 kB
2013-12-25