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Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis
http://hdl.handle.net/2241/91105
http://hdl.handle.net/2241/91105b6fc13e2-0caf-416a-8b52-2fa34e6ea918
名前 / ファイル | ライセンス | アクション |
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DD_236-7.pdf (756.6 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2007-10-02 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
笹倉, 靖徳
× 笹倉, 靖徳 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines, enhancer/gene traps, and insertional mutagenesis. Recently, transposon-mediated transformation using a Tc1/mariner transposable element Minos has been reported in two ascidians: Ciona intestinalis and C. savignyi. The transposon derived from an insect, Drosophila hydei, has high activity for excision in Ciona embryos and transposition in their genome. As much as 37% of Minos-injected C. intestinalis transmitted transposon insertions to the subsequent generation. Minos-mediated germline transgenesis has also been achieved by means of electroporation method. Minos techniques have been applied to enhancer traps and insertional mutagenesis in Ciona. For those reasons, Minos offers the high potential for use as a powerful tool for future genetic studies. This review specifically addresses recent achievements of transformation techniques in Ciona, as exemplified using the Minos system. |
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言語 | en | |||||
書誌情報 |
Developmental dynamics 巻 236, 号 7, p. 1758-1767, 発行日 2007-07 |
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ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1058-8388 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1082160X | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 17342755 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1002/dvdy.21111 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2007 Wiley-Liss, Inc. | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Wiley-Liss |