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Predicting differentiation potential of human pluripotent stem cells: Possibilities and challenges
http://hdl.handle.net/2241/00159172
http://hdl.handle.net/2241/00159172377e2310-1c25-41ea-b45f-26cce8f2e452
名前 / ファイル | ライセンス | アクション |
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WJSC_11-7 (316.7 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2019-12-16 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Predicting differentiation potential of human pluripotent stem cells: Possibilities and challenges | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
鄭, 允文
× 鄭, 允文× Liu, Li-Ping |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The capability of human pluripotent stem cell (hPSC) lines to propagate indefinitely and differentiate into derivatives of three embryonic germ layers makes these cells be powerful tools for basic scientific research and promising agents for translational medicine. However, variations in differentiation tendency and efficiency as well as pluripotency maintenance necessitate the selection of hPSC lines for the intended applications to save time and cost. To screen the qualified cell lines and exclude problematic cell lines, their pluripotency must be confirmed initially by traditional methods such as teratoma formation or by high-throughput gene expression profiling assay. Additionally, their differentiation potential, particularly the lineage-specific differentiation propensities of hPSC lines, should be predicted in an early stage. As a complement to the teratoma assay, RNA sequencing data provide a quantitative estimate of the differentiation ability of hPSCs in vivo. Moreover, multiple scorecards have been developed based on selected gene sets for predicting the differentiation potential into three germ layers or the desired cell type many days before terminal differentiation. For clinical application of hPSCs, the malignant potential of the cells must also be evaluated. A combination of histologic examination of teratoma with quantitation of gene expression data derived from teratoma tissue provides safety-related predictive information by detecting immature teratomas, malignancy marker expression, and other parameters. Although various prediction methods are available, distinct limitations remain such as the discordance of results between different assays and requirement of a long time and high labor and cost, restricting their wide applications in routine studies. Therefore, simpler and more rapid detection assays with high specificity and sensitivity that can be used to monitor the status of hPSCs at any time and fewer targeted markers that are more specific for a given desired cell type are urgently needed. | |||||
書誌情報 |
en : World Journal of Stem Cells 巻 11, 号 7, p. 375-382, 発行日 2019-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1948-0210 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 31396366 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.4252/wjsc.v11.i7.375 | |||||
権利 | ||||||
権利情報 | © The Author(s) 2019 Published by Baishideng Publishing Group Inc. All rights reserved. | |||||
権利 | ||||||
権利情報 | This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ | |||||
著者版フラグ | ||||||
値 | publisher | |||||
出版者 | ||||||
出版者 | Baishideng Publishing Group |