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Unveiling the RNA virosphere associated with marine microorganisms
http://hdl.handle.net/2241/00154145
http://hdl.handle.net/2241/001541452a08d621-1ae8-41e4-852a-7acf60a96617
名前 / ファイル | ライセンス | アクション |
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MER_18-6 (1.8 MB)
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Item type | Journal Article(1) | |||||||||||
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公開日 | 2019-01-22 | |||||||||||
タイトル | ||||||||||||
タイトル | Unveiling the RNA virosphere associated with marine microorganisms | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
資源タイプ | ||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
タイプ | journal article | |||||||||||
著者 |
浦山, 俊一
× 浦山, 俊一
WEKO
204019
× Takaki, Yoshihiro× Nishi, Shinro× Yoshida-Takashima, Yukari× Deguchi, Shigeru× Takai, Ken× Nunoura, Takuro |
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | The study of extracellular DNA viral particles in the ocean is currently one of the most advanced fields of research in viral metagenomic analysis. However, even though the intracellular viruses of marine microorganisms might be the major source of extracellular virus particles in the ocean, the diversity of these intracellular viruses is not well understood. Here, our newly developed method, referred to herein as fragmented and primer ligated dsRNA sequencing (flds) version 2, identified considerable genetic diversity of marine RNA viruses in cell fractions obtained from surface seawater. The RNA virus community appears to cover genome sequences related to more than half of the established positive‐sense ssRNA and dsRNA virus families, in addition to a number of unidentified viral lineages, and such diversity had not been previously observed in floating viral particles. In this study, more dsRNA viral contigs were detected in host cells than in extracellular viral particles. This illustrates the magnitude of the previously unknown marine RNA virus population in cell fractions, which has only been partially assessed by cellular metatranscriptomics and not by contemporary viral metagenomic studies. These results reveal the importance of studying cell fractions to illuminate the full spectrum of viral diversity on Earth. | |||||||||||
書誌情報 |
Molecular ecology resources 巻 18, 号 6, p. 1444-1455, 発行日 2018-09 |
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ISSN | ||||||||||||
収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 1755-098X | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA12362354 | |||||||||||
PubMed番号 | ||||||||||||
識別子タイプ | PMID | |||||||||||
関連識別子 | 30256532 | |||||||||||
DOI | ||||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | 10.1111/1755-0998.12936 | |||||||||||
権利 | ||||||||||||
権利情報 | © 2018 The Authors. Molecular Ecology Resources Published by John Wiley & Sons Ltd | |||||||||||
権利 | ||||||||||||
権利情報 | This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. | |||||||||||
著者版フラグ | ||||||||||||
値 | publisher | |||||||||||
出版者 | ||||||||||||
出版者 | Wiley |